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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of bone morphogenetic protein 9 on osteoblast differentiation of cells grown on titanium with nanotopography

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Author(s):
Souza, Alann T. P. [1] ; Bezerra, Barbara L. S. [1] ; Oliveira, Fabiola S. [1] ; Freitas, Gileade P. [1] ; Bighetti Trevisan, Rayana L. [1] ; Oliveira, Paulo T. [1] ; Rosa, Adalberto L. [1] ; Beloti, Marcio M. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Cellular Biochemistry; v. 119, n. 10, p. 8441-8449, NOV 2018.
Web of Science Citations: 3
Abstract

Among bone morphogenetic proteins (BMPs), BMP-9 has been described as one with higher osteogenic potential. Here, we aimed at evaluating the effect of BMP-9 on the osteoblast differentiation of cells grown on titanium (Ti) with nanotopography, a well-known osseoinductive surface. MC3T3-E1 cells were grown either in absence or presence of BMP-9 (20 nM) on Ti with nanotopography (Ti-Nano) or machined Ti (Ti-Machined) for up to 21 days to evaluate the gene expression of RUNX2, osterix, osteocalcin, bone sialoprotein, SMAD6 and SMAD4, protein expression of SMAD4, ALP activity and extracellular matrix mineralization. As expected BMP-9 increased osteoblast differentiation irrespective of Ti surface topography; however, the cells grown on Ti-Nano were more responsible to BMP-9 compared with cells grown on Ti-machined. This could be, at least in part, due to the fact that Ti-Nano may act on both ways, by increasing the activation (SMAD4) and decreasing the inhibition (SMAD6) of the signaling pathway triggered by BMP-9, while Ti-Machined only decrease the inhibition (SMAD6) of this pathway. In conclusion, the combination of the osteogenic potential of BMP-9 with the osseoinductive capacity of Ti-Nano could be a promising strategy to favor the osseointegration of Ti implants. (AU)

FAPESP's process: 16/14171-0 - Role of Wnt Signaling Pathways in the Osteogenic Potential of Titanium Surface with Nanotopography
Grantee:Márcio Mateus Beloti
Support Opportunities: Regular Research Grants
FAPESP's process: 16/14477-1 - Participation of bone morphogenetic protein 9 (BMP-9) in the interaction between osteoblasts and titanium with nanotopography
Grantee:Barbara Luana da Silva Bezerra
Support Opportunities: Scholarships in Brazil - Scientific Initiation