Menthol ameliorates voiding dysfunction in types I and II diabetic mouse model

Autor(es)

de Oliveira, Mariana G. ^[1] ; Nascimento, Daniel M. ^[1] ; Alexandre, Eduardo C. ^[1] ; Bonilla-Becerra, Sandra M. ^[1] ; Zapparoli, Adriana ^[2] ; Monica, Fabiola Z. ^[1] ; Antunes, Edson ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Med & Expt Surg, Fac Med Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROUROLOGY AND URODYNAMICS; v. 37, n. 8, p. 2510-2518, NOV 2018.
• Citações Web of Science: 4

Resumo

AimsMethodsOveractive bladder (OAB) is one of the most common complications of both type 1 (T1DM) and type 2 diabetes mellitus (T2DM). In healthy conditions, menthol infused intravesically reduces the threshold for initiating micturition reflex, but no study evaluated its effects in diabetic conditions. Therefore, we have used mouse models of T1DM and T2DM to evaluate the effects of menthol on cystometric alterations and increased bladder contractility in vitro. For T1DM induction, male C57BL6 mice were injected with streptozotocin (STZ) and evaluated after 4 weeks. For T2DM induction, mice were fed with high-fat diet (HFD) for 12 weeks to induce obesity. Urodynamic profiles were assessed by filling cystometry through the infusion of menthol (100 mu M for 30min) or vehicle (DMSO 0.1%). Contractile responses to carbachol, potassium chloride (KCl), and electrical-field stimulation (EFS) were measured in isolated bladders after 20min incubation with menthol (100 mu M) or vehicle. ResultsConclusionsFilling cystometry showed that STZ-injected mice exhibited higher bladder capacity, threshold pressure, and non-voiding contractions (NVCs), which were significantly reduced by menthol infusion. The increased voiding frequency in STZ group were unaffected by menthol. In HFD-fed obese mice menthol significantly attenuated the increased threshold pressure and NVC frequency, but unaffected the changes of voiding frequency. In both STZ-injected and HFD-fed mice, incubation of isolated bladders with menthol normalized the enhanced contractile responses to carbachol, KCl, and EFS stimulation. Menthol may be a potential pharmacological option for the treatment of OAB as a consequence of T1DM and T2DM. (AU)

• Processo FAPESP: 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata
• Beneficiário: Edson Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Temático