Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies

Autor(es)

Rodrigues, Eduardo S. ^[1] ; Gomes, Marcos H. F. ^[1] ; Duran, Nadia M. ^[1] ; Cassanji, Joao G. B. ^[1] ; da Cruz, Tatiana N. M. ^[1] ; Sant'Anna Neto, Analder ^[2] ; Savassa, Susilaine M. ^[1] ; de Almeida, Eduardo ^[1] ; Carvalho, Hudson W. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Nucl Instrumentat Lab, Piracicaba - Brazil

^[2] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Forest Sci, Physiol Trees Lab, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 9, NOV 14 2018.
• Citações Web of Science: 6

Resumo

In vivo and micro chemical analytical methods have the potential to improve our understanding of plant metabolism and development. Benchtop microprobe X-ray fluorescence spectroscopy (mu-XRF) presents a huge potential for facing this challenge. Excitation beams of 30 mu m and 1 mm in diameter were employed to address questions in seed technology, phytopathology, plant physiology, and bioremediation. Different elements were analyzed in several situations of agronomic interest: (i) Examples of mu-XRF yielding quantitative maps that reveal the spatial distribution of zinc in common beans (Phaseolus vulgaris) primed seeds. (ii) Chemical images daily recorded at a soybean leaf (Glycine max) infected by anthracnose showed that phosphorus, sulfur, and calcium trended to concentrate in the disease spot. (iii) In vivo measurements at the stem of P vulgaris showed that under root exposure, manganese is absorbed and transported nearly 10-fold faster than iron. (iv) Quantitative maps showed that the lead distribution in a leaf of Eucalyptus hybrid was not homogenous, this element accumulated mainly in the leaf border and midrib, the lead hotspots reached up to 13,400 mg lead kg(-1) fresh tissue weight. These case studies highlight the ability of mu-XRF in performing qualitative and quantitative elemental analysis of fresh and living plant tissues. Thus, it can probe dynamic biological phenomena non-destructively and in real time. (AU)

• Processo FAPESP: 17/16375-4 - Efeitos da aplicação foliar de La e Ce em plantas de soja
• Beneficiário: Eduardo Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado