Evaluation of photodynamic therapy with methylene blue, by the Fourier Transform Infrared Spectroscopy (FT-IR) in Leishmania major - in vitro

Autor(es)

Fagundes, Jaciara ^[1] ; Sakane, Kumiko Koibuchi ^[2] ; Bhattacharjee, Tanmoy ^[3] ; Pinto, Juliana Guerra ^[1] ; Ferreira, Isabelle ^{[1, 4]} ; Raniero, Leandro Jose ^[5] ; Ferreira-Strixino, Juliana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Univap, Photodynam Therapy Lab, Res & Dev Inst, PI&D, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Univap, Infrared Spect Lab, Res & Dev Inst, R&DI, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] Sir John Walsh Res Inst, 310 Great King St, Dunedin 9016 - New Zealand

^[4] UNIP, ICS, Rd Presidente Dutra, Km 157, 5 Rio Comprido, BR-12240420 Sao Jose Dos Campos, SP - Brazil

^[5] Univ Vale Paraiva, Univap, Nanosensors Lab, Res & Dev Inst R&DI, Shishima Hifumi Ave, 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 207, p. 229-235, JAN 15 2019.
• Citações Web of Science: 0

Resumo

Cutaneous Leishmaniasis (CL), a parasitic disease caused by protozoa from the genus Leishmania, affects the skin and mucous membranes. Current treatment modalities have severe side effects, underlining the need for alternative treatments. One of the emerging techniques is Photodynamic Therapy (PDT), wherein a photosensitizer delivered inside pathogen is activated by irradiation, resulting in generation of reactive oxygen species, killing the pathogen. A detailed study of chemical changes within the pathogen due to this therapy may help improve therapeutic efficiency. Therefore, in this study, Fourier Transform Infrared Spectroscopy (FT-IR) was used to investigate the chemical profiles of Leishmania major promastigotes in culture after PDT and compared with untreated pathogens. Results suggest increase in proteins, nuclear material, and disordered and beta-sheet protein structures; and decrease in lipids, carbohydrates, and a-helix and turns, bends protein conformations. The Amide 1 band analysis showed the conformational changes in protein secondary structure. These chemical changes may be associated with known effects of PDT- membrane degradation, and reduction in the energy source of the parasite. (C) 2018 Published by Elsevier B.V. (AU)

• Processo FAPESP: 13/12284-3 - Avaliação da ação da terapia fotodinâmica em Leishmania sp.: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular