Gene expression profile of cytokines produced in biopsies from patients with American cutaneous leishmaniasis

Autor(es)

Carnietto de Hippolito, Daise Damaris ^{[1, 2]} ; Souza Gomes, Aparecida Helena ^[3] ; Maia, Marta Marques ^[1] ; Meira-Strejevitch, Cristina da Silva ^[1] ; Kanamura, Cristina Takami ^[4] ; Lauletta Lindoso, Jose Angelo ^{[5, 6]} ; Pereira-Chioccola, Vera Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Lab Reg Sorocaba, Sao Paulo - Brazil

^[4] Adolfo Lutz Inst, Ctr Patol, Sao Paulo - Brazil

^[5] Inst Infectol Emilio Ribas, Sao Paulo - Brazil

^[6] Hosp Clin Sao Paulo, Fac Med, Lab Soroepidemiol LIM 38, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 189, p. 69-75, JAN 2019.
• Citações Web of Science: 0

Resumo

American cutaneous leishmaniasis (ACL) causes a local inflammatory process, inducing expression of several cytokine genes. Particularly, IFN-gamma can predict to disease susceptibility. Based in these data, this study was aimed to investigate the gene expression profile of IFN-gamma, IL-10, IL-27, TNF-gamma, TGF-beta and IL-6 produced in biopsies from ACL patients; and whether the gene expression profile of IFN-gamma could determine the disease evolution. Gene expression of 6 cytokines was investigated in 40 formalin-fixed paraffin embedded (FFPE) biopsies from patients with cutaneous leishmaniosis (CL); and 10 FFPE biopsies from patients with mucosal leishmaniasis (ML) (control). All 50 patients were infected with Leishmania (Viannia) braziliensis. Gene expression was determined by qPCR; and a normal control group was used for calculations (5 normal biopsies). Values were expressed as Relative Quantification (RQ). The 40 CL patients were classified into 2 groups. CLlowIFN-gamma, 35 patients with RQ for IFN-gamma below 100; and CLhighIFN-gamma, 5 (12.5%) patients with RQ above 100. Significant increase of mRNA levels of IFN-gamma, IL-10 and IL-27 was shown in CLhighIFN-gamma group when compared with CLlowIFN-gamma and ML groups. TNF-alpha levels in CLlowIFN-gamma group were higher than CLhighIFN-gamma and ML groups. TGF-beta and IL-6 were similar in 3 groups. Comparison of cytokine expression/group showed that CLlowIFN-gamma group had an equilibrium between the cytokines analyzed. In ML group, IFN-gamma was over-expressed; but in CLhighIFN-gamma group, besides IFN-gamma, IL-27 was also over-expressed. The immune response to Leishmania induces to identification of some markers, which can be determined by analysis by gene expression of cytokines produced in biopsies. (AU)

• Processo FAPESP: 15/04803-6 - Estudo da expressão de citocinas na modulação da leishmaniose tegumentar americana e a interação com os achados clínico-epidemiológicos e laboratoriais
• Beneficiário: Daise Damaris Carnietto de Hippólito
• Modalidade de apoio: Bolsas no Brasil - Mestrado