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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene expression profile of cytokines produced in biopsies from patients with American cutaneous leishmaniasis

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Author(s):
Carnietto de Hippolito, Daise Damaris [1, 2] ; Souza Gomes, Aparecida Helena [3] ; Maia, Marta Marques [1] ; Meira-Strejevitch, Cristina da Silva [1] ; Kanamura, Cristina Takami [4] ; Lauletta Lindoso, Jose Angelo [5, 6] ; Pereira-Chioccola, Vera Lucia [1]
Total Authors: 7
Affiliation:
[1] Adolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Lab Reg Sorocaba, Sao Paulo - Brazil
[4] Adolfo Lutz Inst, Ctr Patol, Sao Paulo - Brazil
[5] Inst Infectol Emilio Ribas, Sao Paulo - Brazil
[6] Hosp Clin Sao Paulo, Fac Med, Lab Soroepidemiol LIM 38, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Acta Tropica; v. 189, p. 69-75, JAN 2019.
Web of Science Citations: 0
Abstract

American cutaneous leishmaniasis (ACL) causes a local inflammatory process, inducing expression of several cytokine genes. Particularly, IFN-gamma can predict to disease susceptibility. Based in these data, this study was aimed to investigate the gene expression profile of IFN-gamma, IL-10, IL-27, TNF-gamma, TGF-beta and IL-6 produced in biopsies from ACL patients; and whether the gene expression profile of IFN-gamma could determine the disease evolution. Gene expression of 6 cytokines was investigated in 40 formalin-fixed paraffin embedded (FFPE) biopsies from patients with cutaneous leishmaniosis (CL); and 10 FFPE biopsies from patients with mucosal leishmaniasis (ML) (control). All 50 patients were infected with Leishmania (Viannia) braziliensis. Gene expression was determined by qPCR; and a normal control group was used for calculations (5 normal biopsies). Values were expressed as Relative Quantification (RQ). The 40 CL patients were classified into 2 groups. CLlowIFN-gamma, 35 patients with RQ for IFN-gamma below 100; and CLhighIFN-gamma, 5 (12.5%) patients with RQ above 100. Significant increase of mRNA levels of IFN-gamma, IL-10 and IL-27 was shown in CLhighIFN-gamma group when compared with CLlowIFN-gamma and ML groups. TNF-alpha levels in CLlowIFN-gamma group were higher than CLhighIFN-gamma and ML groups. TGF-beta and IL-6 were similar in 3 groups. Comparison of cytokine expression/group showed that CLlowIFN-gamma group had an equilibrium between the cytokines analyzed. In ML group, IFN-gamma was over-expressed; but in CLhighIFN-gamma group, besides IFN-gamma, IL-27 was also over-expressed. The immune response to Leishmania induces to identification of some markers, which can be determined by analysis by gene expression of cytokines produced in biopsies. (AU)

FAPESP's process: 15/04803-6 - Cytokine expression in the modulation of cutaneous leishmaniasis and its interaction with clinical, epidemiological and laboratory findings
Grantee:Daise Damaris Carnietto de Hippólito
Support Opportunities: Scholarships in Brazil - Master