Cytokine expression in the modulation of cutaneous leishmaniasis and its interaction with clinical, epidemiological and laboratory findings

• Grant number: 15/04803-6
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): September 01, 2015
• Effective date (End): August 31, 2017
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Vera Lúcia Pereira Chioccola
• Grantee: Daise Damaris Carnietto de Hippólito
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Leishmaniases are a group of zoonosis caused by protozoa of Leishmania genus with great complexity in biological characteristics. Leishmaniases constitute a serious health public problem worldwide and are widely distributed in all regions of Brazil. In the last years, the cutaneous infection was considered one of most dermatological syndromes diagnosed in travelers that going to endemic areas. The traditional diagnosis of the American cutaneous leishmaniasis (ACL) includes the microscopic examination, determination of the parasite genetic material in biopsy samples. However, as some patients with LTA have a small number of parasites in the lesions, the correct diagnosis is difficult to establish. The evolution of LTA also depends on the host response from the site of the vector bite, location of the lesions and clinical outcome. The cutaneous infection is more usual, which is characterized by a single or few skin ulcers. Around 3% of patients develop the mucosal form, which is characterized by disfiguring lesions, preferably in nasal or oro-pharyngeal mucosa. The host cellular immune response determines the course of the disease. Therefore studies evaluating the disease prognosis could signalize the physician as different therapeutic approaches, avoiding disfigurements that often cause many psychological problems in the affected population. We intend to know whether infected patients are able to express genes for the production of cytokines related to Th1, Th2 or Th17 responses. For the study of gene expression of cytokines (IFN-³, IL-12, IL-4, IL-6, TGF-², IL-27 and IL-17) will be necessary to standardize the RNA extractions, and to evaluate the purity and integrity of the molecules. In parallel we will select candidates for endogenous genes for use in as normalizers in real-time PCR. The candidates for endogenous genes will be: GAPDH gene (glyceraldehyde-3-phosphate dehydrogenase), 18S (ribosomal RNA subunit), B2M (Beta-2 microglobulin), MT-ATP (mitochondrial ATP) UBC (Ubiquitin C) and TBP (TATA Box Binding Protein). These approaches will be investigated in biopsy samples from 50 patients. The results will generate data for a better understanding of the cellular response in patients with ACL.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARNIETTO DE HIPPOLITO, DAISE DAMARIS; SOUZA GOMES, APARECIDA HELENA; MAIA, MARTA MARQUES; MEIRA-STREJEVITCH, CRISTINA DA SILVA; KANAMURA, CRISTINA TAKAMI; LAULETTA LINDOSO, JOSE ANGELO; PEREIRA-CHIOCCOLA, VERA LUCIA. Gene expression profile of cytokines produced in biopsies from patients with American cutaneous leishmaniasis. Acta Tropica, v. 189, p. 69-75, JAN 2019. (15/04803-6)

MAIA, M. M.; MEIRA-STREJEVITCH, C. S.; PEREIRA-CHIOCCOLA, V. L.; DE HIPPOLITO, D. D. C.; SILVA, V. O.; BRANDAO DE MATTOS, C. C.; FREDERICO, F. B.; SIQUEIRA, R. C.; DE MATTOS, L. C.; GRP, FAMERP IAL TOXOPLASMA RES. Evaluation of gene expression levels for cytokines in ocular toxoplasmosis. PARASITE IMMUNOLOGY, v. 39, n. 10, OCT 2017. (13/15879-8, 13/25650-8, 13/10050-5, 14/09496-1, 15/04803-6)

MEIRA-STREJEVITCH, CRISTINA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; MAIA, MARTA MARQUES; CARNIETTO DE HIPPOLITO, DAISE DAMARIS; WANG, HUI-TZU LIN; MOTOIE, GABRIELA; DE SOUZA GOMES, APARECIDA HELENA; KANAMURA, CRISTINA TAKAMI; MARTINES, ROOSECELIS BRASIL; BRANDAO DE MATTOS, CINARA CASSIA; et al. Reference genes for studies in infectious parasitic diseases in five types of human tissues. GENE REPORTS, v. 7, p. 98-105, JUN 2017. (13/15879-8, 13/05811-7, 13/25650-8, 13/10050-5, 14/09496-1, 15/04803-6)

MEIRA-STREJEVITCH, CRISTINA SILVA; PEREIRA, INGRID DE SIQUEIRA; CARNIETTO HIPPOLITO, DAISE DAMARIS; MAIA, MARTA MARQUES; CRUZ, ALLECINEIA BISPO; GAVA, RICARDO; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; MATTOS, LUIZ CARLOS; et al. Ocular toxoplasmosis associated with up-regulation of miR-155-5p/miR-29c-3p and down-regulation of miR-21-5p/miR-125b-5p. CYTOKINE, v. 127, MAR 2020. (15/04803-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HIPPÓLITO, Daise Damaris Carnietto de. Study of genes encoding cytokines involved in the modulation of American cutaneous leishmaniasis and correlation with the clinical-laboratory findings of the disease. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.