Immunohistochemistry profile of p75 neurotrophin receptor in oral epithelial dysplasia and oral squamous cell carcinoma induced by 4-nitroquinoline 1-oxide in rats

Autor(es)

Teixeira Buck, Marina Gabriela ^[1] ; Cabral Tuci, Priscila Souza ^[1] ; Perillo Rosin, Flavia Cristina ^[1] ; Pinheiro Barcessat, Ana Rita ^[2] ; Correa, Luciana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Pathol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Amapa, Sch Nursing, Biol Hlth Sci Dept, Rod Juscelino Kubitschek, BR-68903419 Macapa, AP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 96, p. 169-177, DEC 2018.
• Citações Web of Science: 0

Resumo

Objective: The 4-nitroquinoline 1-oxide (4-NQO) model for carcinogenesis has been used to investigate cancer stem cells (CSC), but no study has addressed the role of the p75 neurotrophin receptor (p75(NTR)) in 4-NQO-induced oral dysplasia and oral squamous cell carcinoma (OSCC). The aim of this study was to evaluate the immunohistochemistry profile of the p75(NTR) during 4-NQO-induced oral carcinogenesis in rats and to verify whether this profile has an association with proliferating cell nuclear antigen (PCNA) immunolabeling. Design: For 28 weeks, rats were exposed to 4-NQO, which was diluted in the drinking water. After 3, 5, 7, 16, and 28 weeks, the animals were euthanized and their tongues were histologically analyzed using p75(NTR) and PCNA immunolabeling. Results: In animals without 4-NQO exposure, the p75(NTR) and PCNA were expressed only in the basal epithelial layer and in a clustered manner. The oral epithelium showed dysplasia and a significant increase in the number of p75(NTR)- and PCNA-positive cells, which were localized mainly in the basal and suprabasal epithelial layers during weeks 5-16 of 4-NQO exposure. When the epithelium invaded the lamina propria and well-differentiated OSCC began, the p75(NTR)-positive cell frequency drastically decreased in epithelial cords and nests, showing a negative correlation with PCNA expression. p75(NTR) immunolabeling during 4-NQO-induced carcinogenesis was similar to that described for human head and neck dysplasia and neoplasia. Conclusions: p75(NTR) immunolabeling observed in 4-NQO-induced oral dysplastic and OSCC lesions were related to the early phases of oral carcinogenesis and may help predict cell dysplasia and malignant transformation. (AU)

• Processo FAPESP: 11/07437-0 - Análise da expressão de marcadores de morte celular em lesões potencialmente malignas induzidas em língua de ratos e tratadas com terapia fotodinâmica
• Beneficiário: Luciana Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular