Isolation of avian nephritis virus from chickens showing enteric disorders

Autor(es)

Nunez, Luis Fabian N. ^{[1, 2]} ; Santander Parra, Silvana H. ^[1] ; De la Torre, David ^{[1, 2]} ; Catroxo, Marcia H. ^[3] ; Buim, Marcos R. ^[4] ; Chacon, Ruy V. ^[1] ; Astolfi Ferreira, Claudete S. ^[1] ; Piantino Ferreira, Antonio J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Av Prof Dr Orlando M Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Cent Univ Ecuador, Sch Vet Med, EC-170521 Quito - Ecuador

^[3] Ctr Res & Dev Anim Hlth, Inst Biol, Lab Electron Microscopy, Av Cons Rodrigues Alves 1252, BR-04014002 Sao Paulo, SP - Brazil

^[4] Inst Biol, Lab Avian Dis, Av Gaspar Ricardo 1700, BR-17690000 Bastos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: POULTRY SCIENCE; v. 97, n. 10, p. 3478-3488, OCT 2018.
• Citações Web of Science: 0

Resumo

Runting-stunting syndrome (RSS) is one of the diseases associated with many detected viruses. In Brazil, there were reports of several enteric disease outbreaks in chickens in which avian nephritis virus (ANV) was detected; however, the role of ANV in the outbreaks and whether the virus was a causative agent of these cases of enteric diseases were not determined. The aim of this study was to isolate ANV in specific pathogen-free (SPF) chicken embryonated eggs (CEE) from the enteric contents of chickens showing signs of RSS. For this purpose, 22 samples of chicken enteric contents that were positive only for ANV were inoculated into 7 and 14-day-old SPF-CEE via the yolk sac route and incubated for 5 d, with a total of 3 passages. Virus isolation was confirmed by the presence of embryo injuries, detection of viral RNA by RT-PCR, and visualization of viral particles using electron microscopy. Therefore, the 7-day-old inoculated embryos showed dwarfism, gelatinous consistency, hemorrhage, and edema in the embryos, whereas the 14-day-old did not show any alteration. Viral RNA was detected in the embryos of both ages of inoculation, and the same viral particles were visualized. The embryos from the mock group showed no alteration and were negative for all the tests. The viral cDNA was sequenced, and the molecular and phylogenetic analyses showed that the Brazilian isolates are more related with the ANV-1 serotype group; the sequences of these isolates showed a high percentage of nucleotide (86.4 to 94.9%) and amino acid (92.3 to 98.7%) similarity with other sequences from China, Japan, Australia, and the United States that belong to this serotype previously classified group. In this study, we isolated 8 samples of ANV in SPF-CEE from enteric content samples from chickens with RSS. In doing so, we showed the pathological injuries to the embryo caused by the virus and the molecular characterization of a part of the ORF 1b gene of the virus. (AU)

• Processo FAPESP: 15/09348-5 - Detecção molecular, quantificação, distribuição tecidual e determinação das alterações patológicas de vírus entéricos relacionados com doença entérica em galinhas.
• Beneficiário: Luis Fabian Nuñez Naranjo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/08560-5 - Estudo da patogenicidade de amostras de vírus entéricos de origem aviária (astrovírus, vírus da nefrite aviária e parvovírus) em aves infectadas experimentalmente
• Beneficiário: Antonio José Piantino Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular