Intracerebellar microinjection of histaminergic compounds on locomotor and exploratory behaviors in mice

Autor(es)

Guilherme, Evelyn M. ^[1] ; Silva-Marques, Bruna ^[1] ; Fernandes, Carlos Eduardo M. ^[1] ; Russo, Thiago L. ^[1] ; Mattioli, Rosana ^[1] ; Gianlorenco, Anna C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Rod Washington Luiz Km 235, BR-13565090 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience Letters; v. 687, p. 10-15, NOV 20 2018.
• Citações Web of Science: 0

Resumo

The neural histaminergic system innervates the cerebellum, with a high density of fibers in the vermis and fiocculus. The cerebellum participates in motor functions, but the role of the histaminergic system in this function is unclear. In the present study, we investigated the effects of intracerebellar histamine injections and H1, H2 and H3 receptor antagonist injections (chlorpheniramine, ranitidine, and thioperamide, respectively) and H4 receptor agonist (VUF-8430) on locomotor and exploratory behaviors in mice. The cerebellar vermis of male mice was implanted with guide cannula. After three days of recovery,the animals received microinjections of saline or histamine (experimentl), saline or chlorpheniramine (experiment 2), saline or ranitidine(experiment 3), saline or thioperamide (experiment 4), and saline or VUF-8430 (experiment 5) in different concentrations. Five minutes postinjection,the open field test was performed. The data were analyzed using one-way ANOVA and Duncan's post hoc test. The microinjections of histamine, ranitidine or thioperamide did not lead any behavioral effects at the used doses. In contrast, animals that received chlorpheniramine at the highest dose (0.16 nmol) and VUF-8430 at the highest dose (1.48 nmol)were more active in the open field apparatus, with an increase in the number of crossed quadrants, number of rearings and time spent in the central area of the arena, suggesting that chlorpheniramine and VUF-8430 modulates locomotor and exploratory behaviors in mice. (AU)

• Processo FAPESP: 16/26071-0 - Influência do sistema histaminérgico cerebelar em funções motoras e não motoras de camundongos
• Beneficiário: Evelyn Maria Guilherme
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/24774-8 - Influência do sistema histaminérgico cerebelar no desempenho motor de camundongos
• Beneficiário: Anna Carolyna Lepesteur Gianlorenço
• Modalidade de apoio: Auxílio à Pesquisa - Regular