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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Kinetic characterization of the gill (Na+, K+)-ATPase in a hololimnetic population of the diadromous Amazon River shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)

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Autor(es):
Fabri, Leonardo M. [1] ; Lucena, Malson N. [2] ; Garcon, Daniela P. [3] ; Moraes, Cintya M. [1] ; McNamara, John C. [4, 5] ; Leone, Francisco A. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Fed Mato Grosso do Sul, BR-79070900 Campo Grande - Brazil
[3] Univ Fed Triangulo Mineiro, BR-38280000 Iturama - Brazil
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Ctr Biol Marinha, BR-11000600 Sao Sebastiao - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 227, p. 64-74, JAN 2019.
Citações Web of Science: 1
Resumo

We provide a kinetic characterization of (Na+, K+)-ATPase activity in a posterior gill microsomal fraction from a hololimnetic population of the diadromous Amazon River shrimp Macrobrachium amazonicum. Sucrose density gradient centrifugation reveals two distinct membrane fractions showing considerable (Na+, K+)ATP-ase activity, but also containing other microsomal ATPases. Only a single immune-reactive (Na+, K+)-ATPase with M-r of approximate to 110 kDa is present that hydrolyzes ATP with V-M = 130.3 +/- 4.8 nmol Pi min(-1) mg protein(-1) and K-0.5 = 0.065 +/- 0.00162 mmol L-1, exhibiting site-site interactions. Stimulation by Na+ (V-M = 127.5 +/- 5.3 nmol Pi min(-1) mg protein(-1), K-0.5 = 5.3 +/- 0.42 mmol L-1), Mg2+ (V-M = 130.6 +/- 6.8 nmol Pi min(-1) mg protein(-1), K-0.5 = 0.33 +/- 0.042 mmol L-1), K+ (V-M = 126.7 +/- 7.7 nmol Pi min(-1) mg protein(-1), K-0.5 = 0.65 +/- 0.0079 mmol L-1) and NH4+ (V-M = 134.5 +/- 8.6 nmol Pi min(-1) mg protein(-1), K-0.5 = 1.28 +/- 0.44 mmol L-1) also obeys cooperative kinetics. Ouabain (K-1 = 0.18 +/- 0.058 mmol L-1) inhibits total ATPase activity by approximate to 70%. This study reveals considerable differences in the kinetic characteristics of the gill (Na+, K+)-ATPase in a hololimnetic population that appear to result from the adaptation of diadromous Macrobrachium amazonicum populations to different limnic habitats. (AU)

Processo FAPESP: 16/25336-0 - Estudo bioquímico comparativo da (Na+, K+)-ATPase branquial de Macrobrachium amazonicum (Heller, 1862) habitantes de regiões costeiras e continentais
Beneficiário:Francisco de Assis Leone
Linha de fomento: Auxílio à Pesquisa - Regular