Endemicity of the High-Risk Clone Klebsiella pneumoniae ST340 Coproducing QnrB, CTX-M-15, and KPC-2 in a Brazilian Hospital

Autor(es):Mostrar menos -

Tolentino, Fernanda Modesto ^{[1, 2, 3]} ; Campagnari Bueno, Maria Fernanda ^[4] ; Franscisco, Gabriela Rodrigues ^[4] ; de Paula Barcelos, Diego Diniz ^[2] ; Lobo, Suzana Margareth ^[2] ; Moreira Batista Tomaz, Francieli Maira ^[2] ; da Silva, Natal Santos ^{[5, 2]} ; de Andrade, Leonardo Neves ^[6] ; Casella, Tiago ^{[2, 7]} ; da Costa Darini, Ana Lucia ^[6] ; Polotto, Milena ^{[1, 2]} ; Garcia, Doroti de Oliveira ^[4] ; Lelles Nogueira, Mara Correa ^[2]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Sao Jose Do Rio Preto - Brazil

^[2] Fac Med Sao Jose Do Rio Preto, Dept Doencas Dermatol Infecciosas & Parasitarias, Ctr Invest Microrganismos, Sao Jose Do Rio Preto - Brazil

^[3] Univ Estadual Paulista, Sao Jose Do Rio Preto - Brazil

^[4] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo - Brazil

^[5] Uniao Fac Grandes Lagos, Lab Modelagens Matemat & Estat Med, Sao Jose Do Rio Preto, SP - Brazil

^[6] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil

^[7] Hosp Base, Lab Cent, Setor Microbiol Clin, Sao Jose Do Rio Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL DRUG RESISTANCE; v. 25, n. 4 DEC 2018.
• Citações Web of Science: 1

Resumo

The dissemination of multiresistant Klebsiella pneumoniae carbapenemase (KPC)-2-producing Klebsiella pneumoniae isolates belonging to international high-risk clones poses a major health care threat. In this study, 48 nonduplicated, carbapenem-resistant K. pneumoniae isolated from 2011 to 2014 in a tertiary hospital were investigated. The bla(KPC-2) gene was the only determinant for carbapenem resistance. The bla(CTX-M-15) gene was the main determinant for the production of extended-spectrum beta-lactamase (ESBL), whereas aph(3 `)-Ia and qnrB were the most common genes associated with resistance to aminoglycosides and quinolones, respectively. Nine different sequence types (STs) were identified. The most common was ST340. Molecular typing by enterobacterial repetitive intergenic consensus-PCR placed 48 strains among 10 different clusters. In the studied hospital, the high-risk clone of KPC-2-producing K. pneumoniae ST340, harboring genes that codify aminoglycoside modifying enzymes, QnrB and CTX-M-15 plus CTXM-2-type ESBLs, is disseminated and acts as a major agent of infections in critically ill patients. (AU)

• Processo FAPESP: 12/21709-5 - Diversidade genética de Klebsiella pneumoniae produtoras de KPC isoladas de diversos hospitais do Estado de São Paulo
• Beneficiário: Doroti de Oliveira Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/17184-0 - Identificação de genes de ESBL, resistência a quinolonas e de virulência em E. coli isoladas de frangos, carne de frango e de pacientes com infecção do trato urinário
• Beneficiário: Mara Corrêa Lelles Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular