Metabolomic Fingerprinting of Salinispora From Atlantic Oceanic Islands

Autor(es)

Bauermeister, Anelize ^{[1, 2]} ; Velasco-Alzate, Karen ^[2] ; Dias, Tiago ^{[3, 4]} ; Macedo, Helena ^{[3, 4]} ; Ferreira, Elthon G. ^[5] ; Jimenez, Paula C. ^{[6, 2]} ; Lotufo, Tito M. C. ^[7] ; Lopes, Norberto P. ^[1] ; Gaudencio, Susana P. ^{[3, 4]} ; Costa-Lotufo, V, Leticia

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, Sao Paulo - Brazil

^[2] V, Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, Sao Paulo - Brazil

^[3] Univ NOVA Lisboa, Fac Ciencias & Tecnol, Lab Biotecnol Azul & Biomed, Dept Quim, UCIBIO REQUIMTE, Caparica - Portugal

^[4] Univ NOVA Lisboa, Fac Ciencias & Tecnol, Lab Biotecnol Azul & Biomed, Dept Ciencias Vida, UCIBIO REQUIMTE, Caparica - Portugal

^[5] Univ Fed Ceara, Dept Quim Organ & Inorgan, Fortaleza, Ceara - Brazil

^[6] Univ Fed Sao Paulo, Dept Ciencias Mar, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Inst Oceanog, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 9, DEC 11 2018.
• Citações Web of Science: 3

Resumo

Salinispora (Micromonosporaceae) is an obligate marine bacterium genus consisting of three species that share over 99% 16S rRNA identity. The genome and biosynthetic pathways of the members of this genus have been widely investigated due to their production of species-specific metabolites. However, despite the species' high genetic similarity, site-specific secondary metabolic gene clusters have been found in Salinispora strains collected at different locations. Therefore, exploring the metabolic expression of Salinispora recovered from different sites may furnish insights into their environmental adaptation or their chemical communication and, further, may lead to the discovery of new natural products. We describe the first occurrence of Salinispora strains in sediments from the Saint Peter and Saint Paul Archipelago (a collection of islets in Brazil) in the Atlantic Ocean, and we investigate the metabolic profiles of these strains by employing mass-spectrometry-based metabolomic approaches, including molecular networking from the Global Natural Products Social Molecular Networking platform. Furthermore, we analyze data from Salinispora strains recovered from sediments from the Madeira Archipelago (Portugal, Macaronesia) in order to provide a wider metabolomic investigation of Salinispora strains from the Atlantic Oceanic islands. Overall, our study evidences a broader geographic influence on the secondary metabolism of Salinispora than was previously proposed. Still, some biosynthetic gene clusters, such as those corresponding to typical chemical signatures of S. arenicola, like saliniketals and rifamycins, are highly conserved among the assessed strains. (AU)

• Processo FAPESP: 17/17648-4 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Anelize Bauermeister
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/17177-6 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático