Volatile phenols are produced by strains of Dekkera bruxellensis under Brazilian fuel ethanol industry-like conditions

Autor(es)

Lima Silva, Lincon Felipe ^[1] ; Reco, Aline Sotta ^[1] ; Pena, Ruben ^[2] ; Ganga, Maria Angelica ^[2] ; Ceccato-Antonini, Sandra Regina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Ctr Ciencias Agr, Dept Tecnol Agroind & Socioecon Rural, Lab Mol & Agr Microbiol, POB 153, BR-13600970 Araras, SP - Brazil

^[2] Univ Santiago Chile, Fac Tecnol, Dept Ciencia & Tecnol Alimentos, Lab Biotechnol & Appl Microbiol, Estn Cent, Obispo Manuel Umana 050, Santiago 9170201 - Chile

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 365, n. 21 NOV 2018.
• Citações Web of Science: 1

Resumo

Dekkera bruxellensis is a spoilage yeast in wine and fuel ethanol fermentations able to produce volatile phenols from hydroxycinnamic acids by the action of the enzymes cinnamate decarboxylase (CD) and vinyphenol reductase (VR) in wine. However, there is no information about this ability in the bioethanol industry. This work evaluated CD and VR activities and 4-ethylphenol production from p-coumaric acid by three strains of D. bruxellensis and PE-2, an industrial Saccharomyces cerevisiae strain. Single and multiple-cycle batch fermentations in molasses and sugarcane juice were carried out. Dekkera bruxellensis strains showed similar CD activity but differences in VR activity. No production of 4-ethylphenol by S. cerevisiae in any fermentation system or media was observed. The concentrations of 4-ethylphenol peaked during active growth of D. bruxellensis in single-cycle fermentation but they were lower than in multiple-cycle fermentation. Higher concentrations were observed in molasses with molar conversion (p-coumaric acid to 4-ethylphenol) ranging from 45% to 85%. As the first report on 4-ethylphenol production in sugarcane musts by D. bruxellensis in industry-like conditions, it opens up a new avenue to investigate its effect on the viability and fermentative capacity of S. cerevisiae as well as to understand the interaction between the yeasts in the bioethanol industry. (AU)

• Processo FAPESP: 16/20680-4 - Produção de etilfenóis por linhagens de Dekkera bruxellensis isoladas da fermentação etanólica: avaliação da atividade enzimática e dos efeitos dos etilfenóis sobre linhagens industriais de Saccharomyces cerevisiae
• Beneficiário: Sandra Regina Ceccato Antonini
• Modalidade de apoio: Auxílio à Pesquisa - Regular