Transcriptomic analyses uncover emerging roles of mucins, lysosome/secretory addressing and detoxification pathways in insect midguts

Autor(es)

Terra, Walter R. ^[1] ; Dias, Renata O. ^[1] ; Oliveira, Pedro L. ^[2] ; Ferreira, Clelia ^[1] ; Venancio, Thiago M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Rio de Janeiro, Inst Bioquim Med, Programa Biotecnol & Biol Mol, Rio De Janeiro - Brazil

^[3] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, Campos Dos Goytacazes - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: CURRENT OPINION IN INSECT SCIENCE; v. 29, p. 34-40, OCT 2018.
• Citações Web of Science: 4

Resumo

The study of insect midgut features has been made possible by the recent availability of transcriptome datasets. These data uncovered the preferential expression of mucus-forming mucins at midgut regions that require protection (e.g. the acidic middle midgut of Musca domestica) or at sites of enzyme immobilization, particularly around the peritrophic membrane of Spodoptera frugiperda. Coleoptera lysosomal peptidases are directed to midgut lumen when over-expressed and targeted to lysosomes by a mechanism other than the mannose 6-phosphate-dependent pathway. We show that this second trend is likely conserved across Annelida, Mollusca, Nematoda, and Arthropoda. Furthermore, midgut transcriptomes of distantly related species reveal a general overexpression of xenobiotic detoxification pathways. In addition to attenuating toxicity of plant-derived compounds and insecticides, we also discuss a role for these detoxification pathways in regulating host-microbiota interactions by metabolizing bacterial secondary metabolites. (AU)

• Processo FAPESP: 17/08103-4 - A digestão nos insetos: uma abordagem molecular, celular, fisiológica e evolutiva
• Beneficiário: Walter Ribeiro Terra
• Modalidade de apoio: Auxílio à Pesquisa - Temático