Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador

Autor(es)

De la Torre, D. ^{[1, 2]} ; Nunez, L. F. N. ^{[1, 2]} ; Puga, B. ^[2] ; Parra, S. H. S. ^[1] ; Astolfi-Ferreira, C. S. ^[1] ; Ferreira, A. J. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Av Prof Orlando Marques de Paiva 87, BR-05588000 Sao Paulo - Brazil

^[2] Cent Univ Ecuador, Sch Vet Med & Anim Sci, Quito - Ecuador

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Poultry Science; v. 20, n. 4, p. 643-650, OCT-DEC 2018.
• Citações Web of Science: 1

Resumo

ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their nucleotide and amino acid sequences were analysed to determine their phylogenetic relationship with the sequences reported in the United States, Canada, China, South Korea, Croatia, Poland, Hungary, and Brazil. Strong similarity of nucleotide and amino acid sequences among all analyzed sequences and between the analysed and reference sequences was demonstrated, and the phylogenetic analysis clustered all the sequences within the same group, demonstrating a strong relation between the studied strains and the reference chicken parvovirus strains. (AU)

• Processo FAPESP: 13/08560-5 - Estudo da patogenicidade de amostras de vírus entéricos de origem aviária (astrovírus, vírus da nefrite aviária e parvovírus) em aves infectadas experimentalmente
• Beneficiário: Antonio José Piantino Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09348-5 - Detecção molecular, quantificação, distribuição tecidual e determinação das alterações patológicas de vírus entéricos relacionados com doença entérica em galinhas.
• Beneficiário: Luis Fabian Nuñez Naranjo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado