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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador

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Author(s):
De la Torre, D. [1, 2] ; Nunez, L. F. N. [1, 2] ; Puga, B. [2] ; Parra, S. H. S. [1] ; Astolfi-Ferreira, C. S. [1] ; Ferreira, A. J. P. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Av Prof Orlando Marques de Paiva 87, BR-05588000 Sao Paulo - Brazil
[2] Cent Univ Ecuador, Sch Vet Med & Anim Sci, Quito - Ecuador
Total Affiliations: 2
Document type: Journal article
Source: Brazilian Journal of Poultry Science; v. 20, n. 4, p. 643-650, OCT-DEC 2018.
Web of Science Citations: 1
Abstract

ABSTRACT Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their nucleotide and amino acid sequences were analysed to determine their phylogenetic relationship with the sequences reported in the United States, Canada, China, South Korea, Croatia, Poland, Hungary, and Brazil. Strong similarity of nucleotide and amino acid sequences among all analyzed sequences and between the analysed and reference sequences was demonstrated, and the phylogenetic analysis clustered all the sequences within the same group, demonstrating a strong relation between the studied strains and the reference chicken parvovirus strains. (AU)

FAPESP's process: 13/08560-5 - Pathogenicity study of chicken enteric viruses (Astrovirus, avian nephritis virus and Parvovirus) in experimentally infected chickens
Grantee:Antonio José Piantino Ferreira
Support Opportunities: Regular Research Grants
FAPESP's process: 15/09348-5 - Molecular detection, quantitation, tissue distribution and determination of pathological changes of enteric viruses related to enteric disease in chickens.
Grantee:Luis Fabian Nuñez Naranjo
Support Opportunities: Scholarships in Brazil - Post-Doctoral