Nitrergic neurotransmission in the paraventricular nucleus of the hypothalamus modulates autonomic, neuroendocrine and behavioral responses to acute restraint stress in rats

Autor(es)

Busnardo, Cristiane ^[1] ; Crestani, Carlos C. ^[2] ; Scopinho, America A. ^[1] ; Packard, Benjamin A. ^[3] ; Resstel, Leonardo B. M. ^[1] ; Correa, Fernando M. A. ^[1] ; Herman, James P. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Nat Act Principles & Toxicol, BR-14800903 Araraquara - Brazil

^[3] Univ Cincinnati, Dept Psychiat & Behav Neurosci, Cincinnati, OH - USA

• Tipo de documento: Artigo Científico
• Fonte: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY; v. 90, p. 16-27, MAR 2 2019.
• Citações Web of Science: 4

Resumo

We investigated the involvement of nitrergic neurotransmission within the paraventricular nucleus of the hypothalamus (PVN) in modulation of local neuronal activation, autonomic and neuroendocrine responses and behavioral consequences of acute restraint stress in rats. Bilateral microinjections of the selective neuronal nitric oxide (NO) synthase (nNOS) inhibitor N-w-Propyl-L-arginine (NPLA) or the NO scavenger carboxy-PTIO into the PVN reduced arterial pressure and heart rate increases, as well as the fall in cutaneous tail temperature induced by restraint stress. PVN injection of either NPLA or carboxy-PTIO also inhibited restraint-induced increases in anxiety-related behaviors in the elevated plus-maze 24 h later. Local microinjection of NPLA or carboxy-PTIO into the PVN reduced the number of c-fos-immunoreactive neurons in the dorsal parvocellular, ventromedial, medial parvocellular and lateral magnocelllular portions of the PVN in animals subjected to restraint stress. However, neither NPLA nor carboxy-PTIO into the PVN affected restraint-induced increases in plasma corticosterone concentration. The present results indicate that PVN nitrergic neurotransmission acting via nNOS activation has a facilitatory influence on autonomic responses to acute restraint and the delayed emotional consequences of restraint stress. Our results also provide evidence of a prominent role of local nitrergic neurotransmission in PVN neuronal activation during stress. (AU)

• Processo FAPESP: 09/05308-8 - Função do núcleo paraventricular do hipotálamo na resposta cardiovascular e neuroendócrina observada em ratos submetidos ao estresse por restrição
• Beneficiário: Cristiane Busnardo Santiago
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/09425-1 - Estudo do envolvimento do mecanismo óxido nítrico-guanilato ciclase no núcleo paraventricular do hipotálamo na ativação neuronal local e na estimulação do eixo hipotálamo-hipófise-adrenal induzidos pelo estresse por restrição agudo em ratos
• Beneficiário: Cristiane Busnardo Santiago
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado