Toward understanding cancer stem cell heterogeneity in the tumor microenvironment

Autor(es)

Bocci, Federico ^{[1, 2]} ; Gearhart-Serna, Larisa ^[3] ; Boareto, Marcelo ^{[4, 5]} ; Ribeiro, Mariana ^[3] ; Ben-Jacob, Eshel ^[1] ; Devi, Gayathri R. ^{[3, 6]} ; Levine, Herbert ^{[1, 7, 8, 9]} ; Onuchic, Jose Nelson ^{[1, 10, 8, 2]} ; Jolly, Mohit Kumar ^{[1, 11]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Rice Univ, Ctr Theoret Biol Phys, Houston, TX 77005 - USA

^[2] Rice Univ, Dept Chem, POB 1892, Houston, TX 77005 - USA

^[3] Duke Univ, Div Surg Sci, Sch Med, Dept Surg, Durham, NC 27710 - USA

^[4] Swiss Inst Bioinformat, CH-1015 Lausanne - Switzerland

^[5] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel - Switzerland

^[6] Duke Canc Inst, Womens Canc Program, Durham, NC 27710 - USA

^[7] Rice Univ, Dept Bioengn, Houston, TX 77005 - USA

^[8] Rice Univ, Dept Phys & Astron, Houston, TX 77005 - USA

^[9] Northeastern Univ, Dept Phys, Boston, MA 02115 - USA

^[10] Rice Univ, Dept Biosci, Houston, TX 77005 - USA

^[11] Indian Inst Sci, Ctr BioSyst Sci & Engn, Bangalore 560012, Karnataka - India

• Tipo de documento: Artigo Científico
• Fonte: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 116, n. 1, p. 148-157, JAN 2 2019.
• Citações Web of Science: 23

Resumo

The epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) formation are two paramount processes driving tumor progression, therapy resistance, and cancer metastasis. Recent experiments show that cells with varying EMT and CSC phenotypes are spatially segregated in the primary tumor. The underlying mechanisms generating such spatiotemporal dynamics in the tumor microenvironment, however, remain largely unexplored. Here, we show through a mechanism-based dynamical model that the diffusion of EMT-inducing signals such as TGF-beta, together with noncell autonomous control of EMT and CSC decision making via the Notch signaling pathway, can explain experimentally observed disparate localization of subsets of CSCs with varying EMT phenotypes in the tumor. Our simulations show that the more mesenchymal CSCs lie at the invasive edge, while the hybrid epithelial/mesenchymal (E/M) CSCs reside in the tumor interior. Further, motivated by the role of Notch-Jagged signaling in mediating EMT and sternness, we investigated the microenvironmental factors that promote Notch-Jagged signaling. We show that many inflammatory cytokines such as IL-6 that can promote Notch-Jagged signaling can (i) stabilize a hybrid E/M phenotype, (ii) increase the likelihood of spatial proximity of hybrid E/M cells, and (iii) expand the fraction of CSCs. To validate the predicted connection between Notch-Jagged signaling and sternness, we knocked down JAG1 in hybrid E/M SUM149 human breast cancer cells in vitro. JAG1 knockdown significantly restricted tumor organoid formation, confirming the key role that Notch-Jagged signaling can play in tumor progression. Together, our integrated computational-experimental framework reveals the underlying principles of spatiotemporal dynamics of EMT and CSCs. (AU)

• Processo FAPESP: 13/14438-8 - Estudo de redes gênicas regulatórias por meio de métodos estocásticos e de mecânica estatística
• Beneficiário: Marcelo Boareto Do Amaral
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto