Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines

Autor(es):Mostrar menos -

da Silva, Paloma L. ^[1] ; Lauretti-Ferreira, Fabiana ^[1] ; de Lima, Maiara Caldas ^[1] ; Lima, Swiany Silveira ^[2] ; Covarrubias, Ambart E. ^[3] ; De Franco, Marcelo ^[4] ; Carvalho, Eneas ^[1] ; Ho, Paulo Lee ^[5] ; da Costa, Renata M. A. ^{[1, 6]} ; Martins, Elizabeth A. L. ^[7] ; Da Silva, Josefa B. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[2] Fundacao Ezequiel Dias, Belo Horizonte, MG - Brazil

^[3] Univ San Sebastian, Sch Med Technol, Fac Hlth Sci, Concepcion - Chile

^[4] Inst Pasteur, Sao Paulo - Brazil

^[5] Inst Butantan, Secao Vacinas Aerob, Sao Paulo - Brazil

^[6] Global Antibiot Res & Dev Partnership GARDP, Drugs Neglected Dis Initiat, Chemin Louis Dunant 15, CH-1202 Geneva - Switzerland

^[7] Inst Butantan, Lab Desenvolvimento Proc, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 19, JAN 7 2019.
• Citações Web of Science: 0

Resumo

BackgroundLeptospirosis is a widespread zoonosis caused by pathogenic prokaryotic microbes of the genus Leptospira. Although there are several reports in the literature, host-pathogen interaction is still poorly understood. The role of chemokine expression is important on the chemotaxis, activation and regulation of immune cells. Recent studies have shown that their expression profiles play an important role on the severity of leptospirosis outcome. We evaluated the phagocytosis of Leptospira by spleens cells from C3H/HeJ, C3H/HePas and BALB/c mouse strains, respectively susceptible, intermediate and resistant to leptospirosis, and by RAW 264.7 macrophages. Besides, we evaluated the effects of CCL2 treatment on the phagocytosis. The cells were incubated with or without CCL2 chemokine, and infected with virulent L. interrogans sv Copenhageni. Cells and culture supernatants were collected for subsequent analysis.ResultsThe number of leptospires was higher in BALB/c cells, CCL2 pre-treated or only infected groups, when compared to C3H/HeJ and C3H/HePas cells. Indeed, CCL2 activation did not interfere in the phagocytosis of Leptospira. Expression of chemokines CXCL5 and CCL8 levels were significantly inhibited in infected BALB/c cells when compared to the non-infected control.ConclusionsHigher ability to phagocytosis and early modulation of some chemokines correlated with the resistance to leptospirosis disease. Exposure to CCL2 did not interfere on phagocytosis of Leptospira in our experimental conditions, but acted in the modulation of chemokines expression during Leptospira infection. (AU)

• Processo FAPESP: 13/16451-1 - Avaliação in vitro da contribuição de CCL2/MCP-1 e a importância de quimiocinas nos mecanismos de resistência e susceptibilidade à infecção por Leptospira sp.
• Beneficiário: Josefa Bezerra da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular