ER PvuII and XbaI polymorphisms in postmenopausal women with posterior tibial tendon dysfunction: a case control study

Autor(es):Mostrar menos -

Pontin, P. A. ^[1] ; Nogara, P. R. B. ^[2] ; Fonseca, F. C. P. ^[1] ; Netto, C. Cesar ^[3] ; Carvalho, K. C. ^[4] ; Soares Junior, J. M. ^[4] ; Baracat, E. C. ^[4] ; Fernandes, T. D. ^[1] ; Maffulli, N. ^{[5, 6, 7]} ; Santos, M. C. L. ^[2] ; Godoy-Santos, A. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Dept Orthopaed & Traumatol, Sao Paulo, SP - Brazil

^[2] Univ Fed Parana, Dept Cell Biol, Curitiba, PR - Brazil

^[3] Hosp Special Surg, Dept Orthoped, 535 E 70th St, New York, NY 10021 - USA

^[4] Univ Sao Paulo, Dept Gynecol, Sao Paulo, SP - Brazil

^[5] Sch Med Surg & Dent, Dept Orthopaed, Salerno - Italy

^[6] Keele Univ, Sch Med, Inst Sci & Technol Med, Stoke On Trent, Staffs - England

^[7] Queen Mary Univ London, Ctr Sports & Exercise Med, Mile End Rd, London E1 4NS - England

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH; v. 13, DEC 11 2018.
• Citações Web of Science: 0

Resumo

BackgroundPosterior tibial tendon (PTT) insufficiency is considered as the main cause of adult acquired flat foot and is three times more frequent in females. High estrogen levels exert a positive effect on the overall collagen synthesis in tendons. We have previously demonstrated the association between some genetic single-nucleotide polymorphism (SNP) and tendinopathy. In the present study, we investigated the association of PvuII c454-397T>C (NCBI ID: rs2234693) and XbaI c454-351A>G (NCBI ID: rs9340799) SNPs in estrogen receptor alfa (ER-) gene with PPT dysfunction.MethodsA total of 92 female subjects with PTT dysfunction, with histopathological examination of the tendon and magnetic resonance image (MRI) evidence of tendinopathy, were compared to 92 asymptomatic females who presented an intact PPT at MRI for PvuII and XbaI SNPs in the ER- gene. Genomic DNA was extracted from saliva and genotypes were obtained by polymerase chain reaction restriction fragment length polymorphism.ResultsThe analysis of PvuII SNPs showed no significant differences in the frequency of alleles and genotypes between control and PTT dysfunction groups. The XbaI SNPs in the ER- gene showed significant differences in the frequency of genotypes between control and test groups (p=0.01; OR 95% 1.14 (0.55-2.33).ConclusionsThe XbaI SNP in the ER gene may contribute to tendinopathy, and the A/A genotype could be a risk factor for PTT tendinopathy in this population. The PvuII SNP studied was not associated with PTT tendinopathy. (AU)

• Processo FAPESP: 15/19635-1 - Análise de polimorfismos nos receptores e na via metabólica de estrogênio, em mulheres pós-menopáusicas com tendinopatia do tibial posterior
• Beneficiário: Alexandre Leme Godoy dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular