Vacuumed collagen-impregnated bioglass scaffolds: Characterization and influence on proliferation and differentiation of bone marrow stromal cells

Autor(es)

Kido, Hueliton Wilian ^[1] ; Gabbai-Armelin, Paulo Roberto ^[1] ; Avanzi, Ingrid Regina ^[1] ; da Silva, Antonio Carlos ^[2] ; Fernandes, Kelly Rossetti ^[1] ; Fortulan, Carlos Alberto ^[3] ; Muniz Renno, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Santos, SP - Brazil

^[2] Nucl & Energy Res Inst IPEN, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Mech Engn, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 107, n. 2, p. 211-222, FEB 2019.
• Citações Web of Science: 2

Resumo

This study evaluated physical-chemical characteristics of a vacuumed collagen-impregnated bioglass (BG) scaffolds and bone marrow stromal cells (BMSCs) behavior on those composites. scanning electron microscope and energy dispersive x-ray spectroscope demonstrated collagen (Col) was successfully introduced into BG. Vacuum impregnation system has showed efficiency for Col impregnation in BG scaffolds (approximately 20 wt %). Furthermore, mass weight decreasing and more stabilized pH were observed over time for BG/Col upon incubation in phosphate buffered saline compared to plain BG under same conditions. Calcium evaluation (Ca assay) demonstrated higher calcium uptake for BG/Col samples compared to BG. In addition, BG samples presented hydroxyapatite crystals formation on its surface after 14 days in simulated body fluid solution, and signs of initial degradation were observed for BG and BG/Col after 21 days. Fourier transform infrared spectroscopy spectra for both groups indicated peaks for hydroxyapatite formation. Finally, a significant increase of BMSCs viability for both composites was observed compared to control group, but no increase of osteogenic differentiation-related gene expressions were found. In summary, BG/Col scaffolds have improved degradation, pH equilibrium and Ca mineralization over time, accompanied by hydroxyapatite formation. Moreover, both BG and BG/Col scaffolds were biocompatible and noncytotoxic, promoting a higher cell viability compared to control. Future investigations should focus on additional molecular and in vivo studies in order to evaluate biomaterial performance for bone tissue engineering applications. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 211-222, 2019. (AU)

• Processo FAPESP: 14/23471-1 - Scaffolds de biovidro/colágeno enriquecidos com células-tronco mesenquimais: efeitos sobre o reparo de defeitos críticos em calvária de ratos
• Beneficiário: Hueliton Wilian Kido
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado