Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction

Autor(es)

Monteiro, Babyla Geraldes ^{[1, 2]} ; Loureiro, Renata Ruoco ^[3] ; Cristovam, Priscila Cardoso ^[3] ; Covre, Joyce Luciana ^[3] ; Pereira Gomes, Jose Alvaro ^[3] ; Kerkis, Irina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Genet, Av Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Oftalmol & Ciencias Visuais, CASO, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Arquivos Brasileiros de Oftalmologia; v. 82, n. 1, p. 32-37, JAN-FEB 2019.
• Citações Web of Science: 1

Resumo

Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction. (AU)

• Processo FAPESP: 10/08595-5 - Estudo do Potencial de Enxerto das Células-tronco Imaturas da Polpa Dentária Humana na Região Límbica após Tratamento da Deficiência Total de Células-tronco Límbicas em Modelo Pré-clínico
• Beneficiário: Bábyla Geraldes Monteiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado