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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Amniotic membrane as a biological scaffold for dental pulp stem cell transplantation in ocular surface reconstruction

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Author(s):
Monteiro, Babyla Geraldes [1, 2] ; Loureiro, Renata Ruoco [3] ; Cristovam, Priscila Cardoso [3] ; Covre, Joyce Luciana [3] ; Pereira Gomes, Jose Alvaro [3] ; Kerkis, Irina [1]
Total Authors: 6
Affiliation:
[1] Inst Butantan, Lab Genet, Av Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Oftalmol & Ciencias Visuais, CASO, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Arquivos Brasileiros de Oftalmologia; v. 82, n. 1, p. 32-37, JAN-FEB 2019.
Web of Science Citations: 1
Abstract

Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Methods: We compared the immunophenotypes (ABCG2, K3/12, and vimentin) of cells grown on amniotic membranes or plastic surfaces under serum-free conditions or in culture media containing serum or serum replacement components. Results: immature dental pulp stem cells grown on amniotic membranes under basal conditions are able to maintain their undifferentiated state. Our data also suggest that the culture medium used in the present work can modulate the expression of immature dental pulp stem cell markers, thus inducing epithelial differentiation of these cells in vitro. Conclusions: Our results suggest that the amniotic membrane is a good choice for the growth and transplantation of mesenchymal stem cells, particularly immature dental pulp stem cells, in clinical ocular surface reconstruction. (AU)

FAPESP's process: 10/08595-5 - Study of Human Immature Dental Pulp Stem Cells Graft Potential in Limbical Region after Transplantation into Total Limbal Stem Cells Deficiency in Animal Model.
Grantee:Bábyla Geraldes Monteiro
Support Opportunities: Scholarships in Brazil - Doctorate