In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep

Autor(es)

Silva Soares, Suelen Carolina ^[1] ; de Lima, Gilmar Cotrin ^[2] ; Laurentiz, Antonio Carlos ^[2] ; Feboli, Aline ^[1] ; dos Anjos, Luciano Alves ^[2] ; de Paula Carlis, Matheus Souza ^[1] ; da Silva Filardi, Rosimeire ^[2] ; da Silva de Laurentiz, Rosangela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Fis & Quim, BR-15385000 Ilha Solteira, SP - Brazil

^[2] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Biol & Zootecnia, BR-15385000 Ilha Solteira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE; v. 6, n. 2, p. 243-247, DEC 2018.
• Citações Web of Science: 1

Resumo

Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep. (AU)

• Processo FAPESP: 14/25294-0 - Avaliação da atividade anti-helmíntica in vitro do bagaço da uva obtido após fermentação para a produção de vinho
• Beneficiário: Suelen Caroline da Silva Soares
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica