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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep

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Author(s):
Silva Soares, Suelen Carolina [1] ; de Lima, Gilmar Cotrin [2] ; Laurentiz, Antonio Carlos [2] ; Feboli, Aline [1] ; dos Anjos, Luciano Alves [2] ; de Paula Carlis, Matheus Souza [1] ; da Silva Filardi, Rosimeire [2] ; da Silva de Laurentiz, Rosangela [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Fis & Quim, BR-15385000 Ilha Solteira, SP - Brazil
[2] Univ Estadual Paulista, Fac Engn Ilha Solteira, Dept Biol & Zootecnia, BR-15385000 Ilha Solteira, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE; v. 6, n. 2, p. 243-247, DEC 2018.
Web of Science Citations: 1
Abstract

Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep. (AU)

FAPESP's process: 14/25294-0 - Evaluation of the in vitro anthelmintic activity of grape pomace obtained after fermentation to produce wine
Grantee:Suelen Caroline da Silva Soares
Support Opportunities: Scholarships in Brazil - Scientific Initiation