Data set of optimal parameters for colorimetric red assay of epoxide hydrolase activity

Autor(es)

deOliveira, Gabriel Stephani ^[1] ; Adriani, Patricia Pereira ^[1] ; Borges, Flavia Garcia ^[1] ; Lopes, Adriana Rios ^[2] ; Campana, Patricia T. ^[1] ; Chambergo, Felipe S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Av Arlindo Bettio 1000, Sao Paulo - Brazil

^[2] Inst Butantan, Av Vital Brasil 1500, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DATA IN BRIEF; v. 8, p. 436-440, SEP 2016.
• Citações Web of Science: 1

Resumo

The data presented in this article are related to the research article entitled ``Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization{''} {[}1]. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to the corresponding vicinal diols. This article describes the optimal parameters for the colorimetric red assay to determine the enzymatic activity, with an emphasis on the characterization of the kinetic parameters, pH optimum and thermal stability of this enzyme. The effects of reagents that are not resistant to oxidation by sodium periodate on the reactions can generate false positives and interfere with the final results of the red assay. (C) 2016 The Authors. Published by Elsevier Inc. (AU)

• Processo FAPESP: 14/24107-1 - Estudo e caracterização de enzimas oxidases produzidas por fungos filamentosos
• Beneficiário: Felipe Santiago Chambergo Alcalde
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 15/03329-9 - Clonagem e caracterização de enzimas epóxido hidrolases de Trichoderma reesei
• Beneficiário: Gabriel Stephani de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto