| Full text | |
| Author(s): |
deOliveira, Gabriel Stephani
[1]
;
Adriani, Patricia Pereira
[1]
;
Borges, Flavia Garcia
[1]
;
Lopes, Adriana Rios
[2]
;
Campana, Patricia T.
[1]
;
Chambergo, Felipe S.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Av Arlindo Bettio 1000, Sao Paulo - Brazil
[2] Inst Butantan, Av Vital Brasil 1500, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | DATA IN BRIEF; v. 8, p. 436-440, SEP 2016. |
| Web of Science Citations: | 1 |
| Abstract | |
The data presented in this article are related to the research article entitled ``Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization{''} {[}1]. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to the corresponding vicinal diols. This article describes the optimal parameters for the colorimetric red assay to determine the enzymatic activity, with an emphasis on the characterization of the kinetic parameters, pH optimum and thermal stability of this enzyme. The effects of reagents that are not resistant to oxidation by sodium periodate on the reactions can generate false positives and interfere with the final results of the red assay. (C) 2016 The Authors. Published by Elsevier Inc. (AU) | |
| FAPESP's process: | 14/24107-1 - Characterization and study of oxidases enzymes from filamentous fungi |
| Grantee: | Felipe Santiago Chambergo Alcalde |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 15/03329-9 - Cloning and characterization of the enzymes epoxide hydrolases from Trichoderma reesei |
| Grantee: | Gabriel Stephani de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |