Recent analysis of the genomic information from several Streptomyces strains has revealed an enormous abundance of gene clusters codifying enzymes from secondary metabolismo. The whole set of information infers that the capability to produce metabolites by Streptomyces and other actinobacteria is underestimated. As alternatives to tradicional screening methodologies, genomic tools representes a promising avenue to isolate new molecules, elucidate biosynthetic pathways and enzymes function and to elucidate biosynthesis intermediates. The endophytic strains Streptomyces wadayamensis and Streptomyces sp. Isolated from Citrus ssp were recently sequenced in our research group using Illumina MiSeq Technology. These strains were choosen after previous studies using metabolic probes to reveal biosynthetic gene clusters and biochemical assays. After sequencing, trimmering and assembly, the genes were annotated and in silico analysis allowed the assignment of the secondary metabolism gene clusters. Exploring the information generated by sequencing we intend to explore diferente methodologies to access known and unknown metabolites: guided incorporation of labeled precursors via genomisotopic approach; gene deletion and metabolite profile evaluation; heterologous expression. Additionally we intend to study and characterize specific enzymes annotated from the genome analysis including terpene cyclases, hydrolases cyclases, monooxygenases and epoxide hydrolases. (AU)
Articles published in Agência FAPESP Newsletter about the research grant:
RAMOS DE JESUS, HUGO CESAR;
RAMOS FERRARI, ALLAN JHONATHAN;
LIMA, DIOGO BORGES;
DREKENER, ROBERTA LOPES;
DUARTE CORREIA, CARLOS ROQUE;
OLIVEIRA, LUCIANA GONZAGA;
DA COSTA NEVES-FERREIRA, ANA GISELE;
CARVALHO, PAULO COSTA;
GOZZO, FABIO CESAR.
XPlex: An Effective, Multiplex Cross-Linking Chemistry for Acidic Residues.
MAY 15 2018.
Web of Science Citations: 6.