Effect of sucrose on biofilm formed in situ on titanium material

Autor(es)

Souza, Joao G. S. ^[1] ; Cury, Jaime A. ^[2] ; Ricomini Filho, Antonio P. ^[2] ; Feres, Magda ^[3] ; de Faveri, Marcelo ^[3] ; Barao, Valentim A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[3] Guarulhos Univ UNG, Dent Res Div, Dept Periodontol, Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 90, n. 2, p. 141-148, FEB 2019.
• Citações Web of Science: 2

Resumo

Background Because sucrose may change the composition of biofilms formed on dental surfaces, the aim of this study was to evaluate in situ the effect of this dietary sugar on biofilm formation on titanium surface. Methods In this blind, crossover, in situ study, 10 volunteers wore, in 3 phases of 7 days each, a palatal appliance containing titanium specimens. In each phase, the specimens were treated extraorally with 20% sucrose solution at a frequency of 4 or 8 times per day. As control, no treatment was rendered (0x). At the end of each phase, the biofilms were collected for biochemical analysis of biofilm wet weight (biomass), protein concentration, soluble (S-EPS), and insoluble (I-EPS) extracellular polysaccharides and intracellular polysaccharides (IPS), and for microbiologic analysis by checkerboard DNA-DNA hybridization (for levels and proportions of 40 bacterial species). Biochemical data were analyzed by linear regression and microbiological findings by Friedman and Dunn tests (alpha = .05). Results A positive significant linear relationship was found among sucrose exposure (0x, 4x, and 8x) and biomass, S-EPS, I-EPS and IPS (p < 0.05). The biofilms treated with sucrose (4x and/or 8x) presented higher mean total levels of the 40 bacterial species evaluated, higher proportions of red complex species and lower proportions of the host-compatible green complex species, in comparison with the control group (p < 0.05). Conclusion The findings of the present study suggest that daily sucrose exposure has a harmful effect on the composition of biofilms formed on titanium surfaces. (AU)

• Processo FAPESP: 15/23118-2 - Efeito antibacteriano e avaliação da biocompatibilidade do tratamento com plasma de descarga incandescente na superfície do titânio: estudo in vitro e in situ
• Beneficiário: João Gabriel Silva Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado