Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex

Autor(es)

Marilena dos Anjos Martins ; Kate Bastos Santos Brighente ^[2] ; Terezinha Aparecida de Matos ^[3] ; Jose Ernesto Vidal ^[4] ; Daise Damaris Carnietto de Hipólito ^[5] ; Vera Lucia Pereira-Chioccola ^[6]

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Infectious Diseases; v. 19, n. 1, p. 62-67, 2015-02-00.

Resumo

Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal fluid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattiiwas observed in the clinical samples of either group. The 695 bp products of C. neoformanswere observed only in 64.6% of the cryptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The specificity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets.Conclusions:These data suggest that the CN4/CN5 primer set was highly sensitive for the identification of C. neoformans/C. gattiispecies complex in cerebrospinal fluid samples from patients with clinical suspicion of cryptococcal meningitis. (AU)

• Processo FAPESP: 11/13939-8 - Estudo de um grupo de infecções fúngico-parasitárias visando a melhoria das técnicas de caracterização e diagnóstico imuno-molecular
• Beneficiário: Vera Lúcia Pereira Chioccola
• Modalidade de apoio: Auxílio à Pesquisa - Regular