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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

IgG reactivity with 40-35 kDa soluble and membrane antigen of Strongyloides venezuelensis in immunocompromised patients

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Autor(es):
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Corral, Marcelo Andreetta [1] ; de Paula, Fabiana Martins [2, 1] ; Meisel, Dirce Mary C. L. [1] ; Abdala, Edson [3] ; Costa, Silvia Figueiredo [3] ; Pierrotti, Ligia Camera [3] ; Yamashiro, Juliana [3] ; do Nascimento Goncalves, Elenice M. [4] ; Castilho, Vera Lucia P. [4] ; Chieffi, Pedro Paulo [5] ; Gryschek, Ronald Cesar B. [2, 1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Hosp Clin, Fac Med, Lab Imunopatol Esquistossomose, Lab Invest Med LIM, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Hosp Clin, Sao Paulo - Brazil
[4] Univ Sao Paulo, Hosp Clin, Fac Med, Secao Parasitol, Div Lab Cent, Sao Paulo - Brazil
[5] Fac Ciencias Med, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Acta Tropica; v. 190, p. 357-360, FEB 2019.
Citações Web of Science: 0
Resumo

Immunocompromised patients constitute a risk group for the development of severe clinical forms of human strongyloidiasis. The diagnosis of this infection is primarily performed by parasitological techniques, but with low sensitivity. Serological techniques appear as an alternative, especially with heterologous antigens use. The aim of this study was to perform the Western blot technique by using S. venezuelensis infective third stage larva (iL3) soluble (TS) and membrane (TM) saline antigens to reveal immunoreactive bands in immunocompromised patients with strongyloidiasis. Serum samples from 117 parasitologically well-characterized patients were divided into four groups: S. stercoralis positive and immunocompetent (S + IC); S. stercoralis positive and immunocompromised (S + IP); negative and immunocompetent (S-IC); negative and immunocompromised (S-IP). A 40-35 kDa band was recognized by 100% of patients in the S + IC group in both antigenic fractions, and by 62.5% and 50% in the S + IP group using the TS and TM fractions, respectively. A 29 kDa band was recognized by 86.3% and 72.7% (for TS and TM, respectively) of patients in the S + IC group, and only by 12.5% of patients in the S + IP group on the TM antigen. Regardless of the patients' immunological condition, the 40-35 kDa band from S. venezuelensis was detected more frequently and can be used as an important marker to the immunodiagnosis of human strongyloidiasis. (AU)

Processo FAPESP: 10/51110-2 - Investigação de técnicas diagnósticas para identificação da infecção por S. stercoralis em pacientes com diferentes formas de imunodepressão
Beneficiário:Pedro Paulo Chieffi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/04236-9 - Imunoproteômica aplicada ao aprimoramento do diagnóstico da estrongiloidíase humana frente a diferentes extratos antigênicos de Strongyloides venezuelensis
Beneficiário:Ronaldo Cesar Borges Gryschek
Linha de fomento: Auxílio à Pesquisa - Regular