Pneumatic Method to Measure Plant Xylem Embolism

Autor(es)

Bittencourt, Paulo R. L. ^{[1, 2, 3]} ; Pereira, Luciano ^[4] ; Oliveira, Rafael S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Exeter, Coll Life & Environm Sci, Exeter, Devon - England

^[2] Univ Estadual Campinas, UNICAMP, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil

^[3] Univ Exeter, Coll Life & Environm Sci, Dept Geog, Amory Bldg, Exeter EX4 4RJ, Devon - England

^[4] Agron Inst IAC, Ctr R&D Ecophysiol & Biophys, Lab Plant Physiol Coaracy M Franco, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIO-PROTOCOL; v. 8, n. 20 OCT 20 2018.
• Citações Web of Science: 0

Resumo

Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning and triggering plant mortality. Measuring embolism with traditional hydraulic methods is both time-consuming and requires large amounts of plant material. While the stem hydraulic methods measure loss of xylem hydraulic conductance due to embolism formation, the pneumatic method directly quantifies the amount of emboli inside the xylem as changes in xylem air content. The pneumatic method is an easy and fast (8+ embolism curves per day) method to measure plant embolism requiring minimal plant material. Here, we provide detailed descriptions and recent technical improvements on the pneumatic method. (AU)

• Processo FAPESP: 11/52072-0 - Interações entre solo-vegetação-atmosfera em uma paisagem tropical em transformação
• Beneficiário: Rafael Silva Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 17/14075-3 - Novos métodos para estudo das propriedades hidráulicas do xilema e embolismo in vivo em plantas
• Beneficiário: Luciano Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado