Antimicrobial photodynamic therapy improves the alveolar repair process and prevents the occurrence of osteonecrosis of the jaws after tooth extraction in senile rats treated with zoledronate

Autor(es):Mostrar menos -

Ervolino, Edilson ^{[1, 2, 3]} ; Statkievicz, Cristian ^[4] ; Toro, Luan Felipe ^{[1, 3]} ; de Mello-Neto, Joao Martins ^{[4, 3]} ; Cavazana, Thamires Priscila ^[5] ; Mardegan Issa, Joao Paulo ^[6] ; Menegati Dornelles, Rita Cassia ^[2] ; de Almeida, Juliano Milanezi ^[4] ; Hitomi Nagata, Maria Jose ^[4] ; Okamoto, Roberta ^{[2, 4]} ; Casatti, Claudio Aparecido ^[2] ; Garcia, Valdir Gouveia ^{[4, 3]} ; Theodoro, Leticia Helena ^{[4, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Grp Res & Study Laser Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Dept Surg & Integrated Clin, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ Unesp, Sch Dent, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[6] Sao Paulo Univ USP, Sch Dent, Dept Morphol Physiol & Basic Pathol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BONE; v. 120, p. 101-113, MAR 2019.
• Citações Web of Science: 3

Resumo

This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNF alpha), interleukin (IL)-1 beta and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction. (AU)

• Processo FAPESP: 13/25367-4 - Efeito do ácido zoledrônico sobre o reparo dos tecido moles sobrejacentes ao sítio de extração dental: estudo envolvendo os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares
• Beneficiário: Thamires Priscila Cavazana
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/03067-6 - Expressão das teneurinas (TEN) e dos peptídeos associados ao terminal carboxila das teneurinas (TCAP) no sistema nervoso central primatas não-humanos (Cebus apella)
• Beneficiário: Cláudio Aparecido Casatti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/26779-4 - Efeito do zoledronato sobre o processo de reparo alveolar: estudo envolvendo os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares e avaliação da terapia fotodinâmica antimicrobiana (aPDT) como proposta preventiva
• Beneficiário: Edilson Ervolino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02199-1 - Avaliação do efeito da Terapia Fotodinâmica Antimicrobiana (aPDT) no processo de reparo alveolar de ratas que apresentam os principais fatores de risco para o desenvolvimento de osteonecrose dos maxilares associada ao uso de bisfosfonatos
• Beneficiário: Cristian Statkievicz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/22395-2 - Avaliação da efetividade e segurança da raspagem e alisamento radicular para tratamento da periodontite experimental em ratos submetidos à terapia com dose oncológica de zoledronato
• Beneficiário: Luan Felipe Toro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica