Encapsulation of S-nitrosoglutathione into chitosan nanoparticles improves drought tolerance of sugarcane plants

Autor(es)

Silveira, Neidiquele M. ^[1] ; Seabra, Amedea B. ^[2] ; Marcos, Fernanda C. C. ^[3] ; Pelegrino, Milena T. ^[2] ; Machado, Eduardo C. ^[1] ; Ribeiro, Rafael V. ^[3]

Afiliação do(s) autor(es)

^[1] Agron Inst IAC, Ctr R&D Ecophysiol & Biophys, Lab Plant Physiol Coaracy M Franco, Campinas, SP - Brazil

^[2] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Lab Crop Physiol, Dept Plant Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 84, p. 38-44, MAR 1 2019.
• Citações Web of Science: 3

Resumo

The entrapment of NO donors in nanomaterials has emerged as a strategy to protect these molecules from rapid degradation, allowing a more controlled release of NO and prolonging its effect. On the other hand, we have found beneficial effects of S-nitrosoglutathione (GSNO) - a NO donor - supplying to sugarcane plants under water deficit. Here, we hypothesized that GSNO encapsulated into nanoparticles would be more effective in attenuating the effects of water deficit on sugarcane plants as compared to the supplying of GSNO in its free form. The synthesis and characterization of chitosan nanoparticles containing GSNO were also reported. Sugarcane plants were grown in nutrient solution, and then subjected to the following treatments: control (well-hydrated); water deficit (WD); WD + GSNO sprayed in its free form (WDG) or encapsulated (WDG-NP). In general, both GSNO forms attenuated the effects of water deficit on sugarcane plants. However, the encapsulation of this donor into chitosan nanoparticles caused higher photosynthetic rates under water deficit, as compared to plants supplied with free GSNO. The root/shoot ratio was also increased when encapsulated GSNO was supplied, indicating that delayed release of NO improves drought tolerance of sugarcane plants. Our results provide experimental evidence that nanotechnology can be used for enhancing NO-induced benefits for plants under stressful conditions, alleviating the negative impact of water deficit on plant metabolism and increasing biomass allocation to root system. (AU)

• Processo FAPESP: 16/10347-6 - Nanopartículas contendo S-nitrosotióis: Síntese, caracterização, ensaios de citotoxicidade e aplicações
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/08194-2 - Óleo essencial contendo nanopartículas metálicas funcionalizadas com óxido nítrico como estratégia para o controle de patógenos vegetais na agricultura
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/19167-0 - Envolvimento do óxido nítrico nas respostas fisiológicas em plantas de cana-de-açúcar submetidas a déficit hídrico
• Beneficiário: Neidiquele Maria Silveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/00393-8 - Nanopartículas poliméricas doadoras de óxido nítrico para aplicações tópicas
• Beneficiário: Milena Trevisan Pelegrino
• Modalidade de apoio: Bolsas no Brasil - Mestrado