| Texto completo | |
| Autor(es): |
dos Santos, Angelica Carreira
[1]
;
Ribeiro, Daniel Araki
[2]
;
da Cruz Ferreira, Jessica Almeida
[3]
;
Aguiar, Odair
[2]
;
Waitzberg, Dan Linetzky
[4]
;
Alves, Claudia Cristina
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med, Dept Pediat, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biosci, Santos - Brazil
[3] Univ Fed Sao Paulo, Santos - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NUTRITION & FOOD SCIENCE; v. 49, n. 1, p. 75-84, FEB 11 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Purpose The purpose of this study is to evaluate the effects of prebiotic, probiotic and synbiotic supplementation on liver histopathology and TLR-4, NF kappa B and TNF-alpha gene expression involved in the inflammatory cascade and pathogenesis of experimental nonalcoholic fatty liver disease (NAFLD). Design/methodology/approach Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (60 days). On Day 30 of hypercholesterolemic conditions, rats were subdivided in five groups: negative control (NC), positive control (PC), prebiotic (PRE), probiotic (PRO) and synbiotic (SYN). All rats were sacrificed on Day 60. Liver tissue was used to verify histopathological changes and gene expression. Gene expression of TLR-4, TNF-alpha and NF kappa B was evaluated in liver tissue using RT-qPCR. Findings Histopathological analysis: PC showed more changes than NC, and PRE and SYN showed fewer alterations than PC. Gene expression analysis: PRE showed higher TLR-4, and NF kappa B and TNF-alpha compared to PC. Also, PRE showed higher TLR-4 when compared to PRO and SYN. SYN group revealed higher TLR-4 and NF kappa B expressions compared to PC. PRO group also showed higher NF kappa B expression compared to PC. Originality/value NAFLD is a significant health concern, and it found that prebiotic, probiotic and synbiotic supplementation could have positive effects as a nonpharmacological approach to control this disease. (AU) | |
| Processo FAPESP: | 17/18524-7 - Prebiotic and Symbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression After Experimental Hypercholesterolemia in Rat Liver |
| Beneficiário: | Claudia Cristina Alves Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Publicações científicas - Artigo |
| Processo FAPESP: | 11/50289-1 - Influência da administração de prebióticos e probióticos na histopatologia e expressão gênica de fatores reguladores da lipogênese e beta-oxidação hepática em ratos hipercolesterolêmicos |
| Beneficiário: | Claudia Cristina Alves Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |