Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images

Autor(es)

da Silva, Nubia Rosa ^{[1, 2]} ; da Silva Oliveira, Marcos William ^{[1, 3]} ; de Almeida Filho, Humberto Antunes ^[3] ; Souza Pinheiro, Luiz Felipe ^{[4, 5]} ; Kolb, Rosana Marta ^{[4, 5]} ; Bruno, Odemir Martinez ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Math & Comp Sci, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Fed Goias, UFG, Inst Biotechnol, Av Dr Lamartine Pinto de Avelar 1200, BR-75704020 Catalao, Go - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, Av Trabalhador Sao Carlense 400, BR-13560970 Sao Carlos, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Sch Sci Humanities & Languages, Dept Biol Sci, Av Dom Antonio 2100, BR-19806900 Assis, SP - Brazil

^[5] Sao Paulo State Univ, UNESP, Biosci Inst, Av 24A, 1515, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIO-PROTOCOL; v. 7, n. 23 DEC 5 2017.
• Citações Web of Science: 1

Resumo

The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of texture image analysis are used to extract characteristics from samples of imaged leaves and thus predict the species. Texture image analysis is a versatile and powerful technique able to extract measurements from patterns in the images. Using this technique, recent research has found a close relationship between texture and plant species (da Silva et al., 2015 and 2016). Here, we describe the procedure to extract texture features from microscopic images of leaves using Fourier (Cosgriff, 1960; Azencott, 1997; Gonzalez and Woods, 2006). It is important to highlight that other methods for texture extraction can be used as well. This protocol is split into two parts: (A) leaf epidermal dissociation and (B) automatic method for leaf epidermal image analysis. (AU)

• Processo FAPESP: 11/23112-3 - Anatomia foliar de plantas de diferentes formações vegetacionais
• Beneficiário: Rosana Marta Kolb
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/01523-1 - Métodos de visão computacional aplicados à identificação e análise de plantas
• Beneficiário: Odemir Martinez Bruno
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/21467-9 - Reconhecimento de Padrões Heterogêneos e suas Aplicações em Biologia e Nanotecnologia.
• Beneficiário: Núbia Rosa da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado