Effect of Er:YAG laser irradiation and chitosan biomodification on the stability of resin/demineralized bovine dentin bond

Autor(es)

Curylofo-Zotti, Fabiana Almeida ^[1] ; Salles Scheffel, Debora Lopes ^[2] ; Macedo, Ana Paula ^[3] ; de Souza-Gabriel, Aline Evangelista ^[1] ; Hebling, Josimeri ^[2] ; Milori Corona, Silmara Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Restorat Dent, Ribeirao Preto Sch Dent, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] UNFSP Univ Estadual Paulista, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Sch Dent, Dept Dent Mat & Prosthodont, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 91, p. 220-228, MAR 2019.
• Citações Web of Science: 0

Resumo

Objective: The purpose of this study was to evaluate the effect of Er:YAG laser for selective removal of carious lesion, followed by biomodification with chitosan on the microtensile bond strength (mu TBS), adhesive interface, dry mass loss and hydroxyproline release (HYP). Methods: Artificial lesions were created in 104 bovine dentin blocks. Blocks were divided according to caries removal method: bur or Er:YAG laser. Seventy-six blocks were acid etched and subdivided according to dentin biomodification: no chitosan and 2.5% chitosan. Composite resin restorations were performed. Blocks were sectioned into beams and stored in water. After 24 h, 6 and 12 months, beams were submitted to mu TBS test (n = 10) and analysis of adhesive interface by SEM (n = 3). The other 28 blocks were sectioned into beams and initial dry mass (DM) was determined (n = 7). Beams were stored and after 7 days, DM was redetermined. HYP release (n = 7) was evaluated by ELJSA. Data were analyzed by ANOVA and Bonferroni's tests (alpha = 0.05). Results: After 24 h, the highest mu TBS was found for bur (p < 0.001). After 6 months, methods were similar (p = 0.432). After 12 months, laser-irradiated dentin showed the highest mu TBS values (p = 0.025). Chitosan promoted higher mu TBS values after 6 (p = 0.011) and 12 months (p < 0.001) preserving adhesive interface. Dry mass loss and HYP release were not influenced (p > 0.05) by caries removal method or by dentin biomodification. Conclusion: The bond strength to demineralized dentin reduced over 50% in all groups after water storage. From 6 months of water storage, Er:YAG laser irradiation and biomodification with chitosan maintained the stability of the resin-dentin bonds, but did not influence dry mass loss and HYP release. (AU)

• Processo FAPESP: 14/23654-9 - Remoção seletiva de lesão de cárie com Laser de Er:YAG e reumidificação da dentina com solução de quitosana
• Beneficiário: Fabiana Almeida Curylofo Zotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado