Silk fibroin hydrogels for potential applications in photodynamic therapy

Autor(es)

Rojas, Jose Eduardo U. ^[1] ; Gerbelli, Barbara B. ^[1] ; Ribeiro, Anderson O. ^[1] ; Nantes-Cardoso, Iseli L. ^[1] ; Giuntini, Francesca ^[2] ; Alves, Wendel A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil

^[2] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool, Merseyside - England

• Tipo de documento: Artigo Científico
• Fonte: Biopolymers; v. 110, n. 2 FEB 2019.
• Citações Web of Science: 1

Resumo

In this study, we prepared translucid hydrogels with different concentrations of silk fibroin, extracted from raw silk fibers, and used them as a matrix to incorporate the photosensitizer 5-(4-aminophenyl)-10,15,20-tris-(4-sulphonatophenyl) porphyrin trisodium for application in photodynamic therapy (PDT). The hydrogels obtained were characterized by rheology, spectrophotometry, and scattering techniques to elucidate the factors involved in the formation of the hydrogel, and to characterize the behavior of silk fibroin (SF) after incorporating of the porphyrin to the matrix. The rheology results demonstrated that the SF hydrogels had a shear thinning behavior. In addition, we were able to verify that the structure of the material was able to be recovered over time after shear deformation. The encapsulation of porphyrins in hydrogels leads to the formation of self-assembled peptide nanostructures that prevent porphyrin aggregation, thereby greatly increasing the generation of singlet oxygen. Also, our findings suggest that porphyrin can diffuse out of the hydrogel and permeate the outer skin layers. This evidence suggests that SF hydrogels could be used as porphyrin encapsulation and as a drug carrier for the sustained release of photosensitizers for PDT. (AU)

• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
• Beneficiário: Adalberto Fazzio
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
• Beneficiário: Wendel Andrade Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/18527-8 - Ftalocianinas e Naftalocianinas: síntese de macrociclos anfifílicos para aplicação em Terapia Fotodinâmica
• Beneficiário: Anderson Orzari Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50972-1 - Francesca Giuntini | Liverpool John Moores University - Inglaterra
• Beneficiário: Wendel Andrade Alves
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional