Polyphasic approach using multilocus analyses supports the establishment of the new aerophytic cyanobacterial genus Pycnacronema (Coleofasciculaceae, Oscillatoriales)

Autor(es)

Martins, Mariellen Dornelles ^[1] ; Machado-de-Lima, Nathali Maria ^[2] ; Zanini Branco, Luis Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Bot & Zool, Sao Jose do Rio Preto Campus IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Bot & Zool, Microbiol Grad Program IBILCE UNESP, Sao Jose do Rio Preto Campus IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYCOLOGY; v. 55, n. 1, p. 146-159, FEB 2019.
• Citações Web of Science: 2

Resumo

A new Phormidium-like genus was found during an investigation of Oscillatoriales diversity in Brazil. Eight aerophytic populations from south and southeastern regions were isolated in monospecific cultures and submitted to polyphasic evaluation. The populations presented homogeneous morphology with straight trichomes, not attenuated, and apical cell with thickened cell wall. Phylogenetic analyses based on 16S rRNA gene sequences showed that these populations, plus the Brazilian strain Phomidium sp. B-Tom from GenBank, formed a highly supported and distinctive clade, which corresponds to the new genus Pycnacronema, comprising six new species: P. brasiliensis (type species), P. arboriculum, P. conicum, P. marmoreum, P. rubrum, and P. savannensis. These results were confirmed and supported by rpoC1 and rbcL genes evaluated independently and by the concatenated analysis of 16S rRNA, rpoC1 and rbcL genes (for all species but P. savannensis). Secondary structures of the D1-D1 `, box-B, and V3 regions of the internal transcribed spacer were informative at specific level, being conserved in P. brasiliensis and variable among the other strains, also confirming the phylogenetic analyses. The generic name and specific epithets of the new taxa are proposed under the provisions of the International Code of Nomenclature of algae, fungi, and plants. (AU)

• Processo FAPESP: 10/09686-4 - Estudos polifásicos de populações de Phormidioideae (Oscillatoriales, Cyanobacteria)
• Beneficiário: Mariéllen Dornelles Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/19468-0 - Perfil molecular e diversidade taxonômica de assembléias de cianobactérias em crostas biológicas de solos de cerrado
• Beneficiário: Luis Henrique Zanini Branco
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08207-3 - Estudos de identificação e de filogenia de Phormidioideae (Oscillatoriales, Cyanobactéria)
• Beneficiário: Mariéllen Dornelles Martins
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado