Polyphasic approach using multilocus analyses supports the establishment of the new aerophytic cyanobacterial genus Pycnacronema (Coleofasciculaceae, Oscillatoriales)

Author(s)

Martins, Mariellen Dornelles ^[1] ; Machado-de-Lima, Nathali Maria ^[2] ; Zanini Branco, Luis Henrique ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Dept Bot & Zool, Sao Jose do Rio Preto Campus IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Bot & Zool, Microbiol Grad Program IBILCE UNESP, Sao Jose do Rio Preto Campus IBILCE, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHYCOLOGY; v. 55, n. 1, p. 146-159, FEB 2019.
• Web of Science Citations: 2

Abstract

A new Phormidium-like genus was found during an investigation of Oscillatoriales diversity in Brazil. Eight aerophytic populations from south and southeastern regions were isolated in monospecific cultures and submitted to polyphasic evaluation. The populations presented homogeneous morphology with straight trichomes, not attenuated, and apical cell with thickened cell wall. Phylogenetic analyses based on 16S rRNA gene sequences showed that these populations, plus the Brazilian strain Phomidium sp. B-Tom from GenBank, formed a highly supported and distinctive clade, which corresponds to the new genus Pycnacronema, comprising six new species: P. brasiliensis (type species), P. arboriculum, P. conicum, P. marmoreum, P. rubrum, and P. savannensis. These results were confirmed and supported by rpoC1 and rbcL genes evaluated independently and by the concatenated analysis of 16S rRNA, rpoC1 and rbcL genes (for all species but P. savannensis). Secondary structures of the D1-D1 `, box-B, and V3 regions of the internal transcribed spacer were informative at specific level, being conserved in P. brasiliensis and variable among the other strains, also confirming the phylogenetic analyses. The generic name and specific epithets of the new taxa are proposed under the provisions of the International Code of Nomenclature of algae, fungi, and plants. (AU)

• FAPESP's process: 10/09686-4 - Polyphasic studies on Phormidioideae (Oscillatoriales, cyanobacteria) populations
• Grantee: Mariéllen Dornelles Martins
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/19468-0 - Molecular profile and taxonomic diversity of cyanobacterial assemblages in biological soil crusts from Brazilian Savanna areas
• Grantee: Luis Henrique Zanini Branco
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/08207-3 - Identification and phylogenetic studies of Phormidioideae (Oscillatoriales, cyanobacteria)
• Grantee: Mariéllen Dornelles Martins
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate