Heparanase-1 activity and the early postnatal prostate development

Autor(es)

Barbosa, Guilherme Oliveira ^[1] ; Bruni-Cardoso, Alexandre ^[2] ; da Silva Pinhal, Maria Aparecida ^[3] ; Augusto, Taize Machado ^[4] ; Carvalho, Hernandes F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biol Estrut & Func, Inst Biol, Campinas, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Butanta, SP - Brazil

^[3] Fundacao ABC, Fac Med, Santo Andre, SP - Brazil

^[4] Fac Med Jundiai, Dept Clin, Jundiai, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DEVELOPMENTAL DYNAMICS; v. 248, n. 3, p. 211-220, MAR 2019.
• Citações Web of Science: 1

Resumo

Ventral prostate (VP) morphogenesis starts during embryonic development and continues for the first three postnatal weeks. Heparan sulfate (HS) affects paracrine signaling. Heparanase-1 (HPSE) is the only enzyme capable of cleaving HS. HPSE releases the HS bioactive fragment and mobilizes growth factors. Little is known, however, about HS turnover and HPSE function during VP morphogenesis. In this study, we measured HSPG expression and analyzed the expression and distribution of HPSE in the rat VP. HPSE was predominantly expressed by the VP epithelium. The VP was treated with heparin in ex vivo cultures to interfere with HS and resulted in delayed epithelial growth. Hpse knockdown using siRNA delayed epithelial growth in the first postnatal week ex vivo, which was similar to treating with the lower concentration of heparin. Hpse silencing was related to changes in HS chain length (as determined by size-exclusion chromatography, up-regulation of Mmp9, and down-regulation of Mmp2 expression). It also down-modulated ERK1/2 phosphorylation, suggesting a reduction in signaling, likely due to decreased HS cleavage and growth factor bioavailability. Our results showed that HPSE played a role in early epithelial growth during the first week of VP postnatal development. Developmental Dynamics 248:211-220, 2019. (c) 2019 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 12/17657-0 - Atividade da heparanase e seu papel na modulação da sinalização extracelular no desenvolvimento prostático
• Beneficiário: Guilherme Oliveira Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/16150-6 - Regulação androgênica, sinalização e interações celulares no desenvolvimento, fisiologia e regressão prostática
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático