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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Immunosenescence in honey bees (Apis mellifera L.) is caused by intrinsic senescence and behavioral physiology

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Autor(es):
Lourenco, Anete Pedro [1, 2] ; Martins, Juliana Ramos [1] ; Silva Torres, Fernanda Andrade [3, 1] ; Mackert, Aline [3, 4] ; Aguiar, Luiz Roberto [1] ; Hartfelder, Klaus [5] ; Gentile Bitondi, Marcia Maria [3] ; Paulino Simoes, Zila Luz [3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Fed Vales Jequitinhonha & Mucuri, Dept Ciencias Biol, Fac Ciencias Biol & Saude, Campus JK, Rodovia MGT 367, Km 583, BR-39100000 Diamantina, MG - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil
[4] Univ Fed Mato Grosso do Sul, Dept Ciencias Ambiente, Campus Pantanal, Corumba, MS - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Experimental Gerontology; v. 119, p. 174-183, MAY 2019.
Citações Web of Science: 1
Resumo

Young honey bee workers (0 to 2-3 weeks old) perform tasks inside the colony, including brood care (nursing), whereas older workers undergo foraging tasks during the next 3-4 weeks, when an intrinsic senescence program culminates in worker death. We hypothesized that foragers are less able to react to immune system stimulation than nurse bees and that this difference is due to an inefficient immune response in foragers. To test this hypothesis, we used an experimental design that allowed us to uncouple chronological age and behavior status (nursing/foraging). Worker bees from a normal age demography colony (where workers naturally transit from nursing to foraging tasks as they age) and of a single-cohort colony setup (composed of same-aged workers performing nursing or foraging tasks) were tested for survival and capability of activation of the immune system after bacterial injection. Expression of an antimicrobial peptide gene, defensin-1 (def-1), was used to assess immune system activation. We then checked whether the immune response includes changes in the expression of aging- and behavior-related genes, specifically vitellogenin (vg), juvenile hormone esterase (jhe), and insulin-like peptide-1 (ilp-1). We found a significant difference in survival rate between bees of different ages but carrying out the same tasks. Our results thus indicate that the bees' immune response is negatively affected by intrinsic senescence. Additionally, independent of age, foragers had a shorter lifespan than nurses after bacterial infection, although both were able to induce def-1 transcription. In the normal age demography colony, the immune system activation resulted in a reduction in the expression of vg, jhe and ilp-1 genes in foragers, but not in the nurse bees, demonstrating that age and behavior are both important influences on the bees' immune response. By disentangling the effects of age and behavior in the single-cohort colony, we found that vg, jhe and ilp-1 response to immune system stimulation was independent of behavior. Younger bees were able to mount a stronger immune response than older bees, thus highlighting age as an important factor for immunity. Taken together, our results provide new insights into how age and behavior affect the honey bee's immune response. (AU)

Processo FAPESP: 04/00732-2 - Biologia molecular de genes envolvidos no metabolismo do hormônio juvenil em Apis mellifera
Beneficiário:Aline Mackert dos Santos
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 05/03302-1 - Hexamerina hex 70c de Apis mellifera: caracterização do cDNA e expressão durante a metamorfose e reprodução
Beneficiário:Juliana Ramos Martins
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 03/07041-2 - Estudo dos genes codificadores dos peptídeos antimicrobianos e de outros genes efetores na resposta imune em Apis mellifera
Beneficiário:Anete Pedro Lourenço
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/03171-5 - Análise causal do desenvolvimento de Apis mellifera - genes reguladores e redes hierárquicas de expressão gênica na especificação de tecidos e órgãos
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/03926-5 - Genômica funcional de Apis mellifera: busca de novos genes e redes funcionais no contexto do desenvolvimento, da diferenciação de castas e da reprodução
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 03/10581-9 - Clonagem do cDNA do hormônio protoracicotropico (PTTH), estudo de sua expressão e hibridação in situ em rainhas e operárias de Apis melifera
Beneficiário:Fernanda Andrade Silva Torres
Linha de fomento: Bolsas no Brasil - Doutorado Direto