Genome sequence of the H-2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant

Autor(es)

Fonseca, Bruna Constants ^[1] ; Riano-Pachon, Diego Mauricio ^{[2, 3]} ; Guazzaroni, Maria-Eugenia ^[4] ; Reginatto, Valeria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Biotecnol Ambiental & Energias Renovaveis LAB, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Lab Biol Sistemas Regulatorios, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Biol Computac Evolut & Sistemas, Av Centenario 303, BR-13416000 Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 1, p. 139-144, JAN-MAR 2019.
• Citações Web of Science: 0

Resumo

We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the chemical block 1,3-propanediol, which is used to obtain polymers. Moreover, the genome of strain Br21 has a higher number of ORFs with predicted beta-glucosidase activity as compared to other C. beijerinckii strains described in the KEGG database. These characteristics make C. beijerinckii strain Br21 a remarkable candidate for direct use in biotechnological processes and attest that it is a potential biocatalyst supplier. (AU)

• Processo FAPESP: 15/04309-1 - Novas abordagens para melhorar a prospecção funcional de biocatalizadores em bibliotecas metagenômicas
• Beneficiário: María Eugenia Guazzaroni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 15/06074-1 - Produção de biohidrogênio de terceira geração a partir de biomassa de alga e seus derivados de hidrólise
• Beneficiário: Valeria Reginatto Spiller
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular