Genome sequence of the H-2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant

Author(s)

Fonseca, Bruna Constants ^[1] ; Riano-Pachon, Diego Mauricio ^{[2, 3]} ; Guazzaroni, Maria-Eugenia ^[4] ; Reginatto, Valeria ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Biotecnol Ambiental & Energias Renovaveis LAB, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Lab Biol Sistemas Regulatorios, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Biol Computac Evolut & Sistemas, Av Centenario 303, BR-13416000 Piracicaba, SP - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 1, p. 139-144, JAN-MAR 2019.
• Web of Science Citations: 0

Abstract

We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the chemical block 1,3-propanediol, which is used to obtain polymers. Moreover, the genome of strain Br21 has a higher number of ORFs with predicted beta-glucosidase activity as compared to other C. beijerinckii strains described in the KEGG database. These characteristics make C. beijerinckii strain Br21 a remarkable candidate for direct use in biotechnological processes and attest that it is a potential biocatalyst supplier. (AU)

• FAPESP's process: 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries
• Grantee: María Eugenia Guazzaroni
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 15/06074-1 - Third-generation biohydrogen production from algae biomass and its hydrolysis derivatives
• Grantee: Valeria Reginatto Spiller
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants