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Applied metagenomics for biofuels development and environmental sustainability in biorefineries based on sugarcane

Grant number: 15/21650-9
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): April 01, 2016
Effective date (End): March 31, 2017
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Marcelo Zaiat
Grantee:Antônio Djalma Nunes Ferraz Júnior
Supervisor: Eric Trably
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
Research place: Institut National de la Recherche Agronomique, Narbonne (INRA), France  
Associated to the scholarship:13/15665-8 - Structure and functional dynamics of microbial community of anaerobic digesters applied to energy recovery and environmental framework of wastes generated in bioethanol production of 1st and 2nd generation, BP.PD


During the bioethanol production process, different liquid and solid waste streams emerge, which hold the potential for its exploitation in a biorefinery process. Thus, waste streams can be treated by a simultaneous production of value added components additionally to bioethanol making the overall process more sustainable. In this scenario, anaerobic digestion of vinasse (the most abundant liquid waste in a biorefinery) arises as an interesting alternative, since in addition of promoting stabilization of organic matter, it also enables energy generation from biogas. Few studies have reported the potential to produce hydrogen and methane using sugarcane vinasse as a substrate. Most of them have covered a selection of the more appropriate type of reactor and operational conditions to the liquid waste in question, based on parameters of quality (organic matter conversion and values of production and yield of biogas), leaving a gap in relation to the studies of gas producing microbial communities. The application of metagenomic techniques can improve the overall performance of the anaerobic digestion in sugarcane-based biorefineries plants. In this plan is proposed the research internship at French National Institute for Agriculture Research (INRA) to assess the structural and functional dynamics of microbiomes in digesters producing hydrogen and methane from sugarcane vinasse, based on next-generation sequencing (NGS) using MiSeq Sequencing System (Illumina) technique. Highlighting the acidogenic biomass, 16S rDNA fingerprints, characterization of functional genes (hydA) and the development of probes for PCRq of homoacetogens will be performed. Still, techniques of how to regulate the homoacetogenic bacteria (HoA) will also be performed. Then, the best regulation condition will be applied in a hydrogen-producing system (CSTR) in order to obtain a balance between HPB and HoA, consequently, continuous hydrogen production. Such techniques focused on open cultures (non-sterile mixed cultures, microbiome) in anaerobic digesters will be used for the posterior application at CTBE/CNPEM after returning from the internship abroad. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NUNES FERRAZ JUNIOR, ANTONIO DJALMA; PAGES, CLEMENCE; LATRILLE, ERIC; BERNET, NICOLAS; ZAIAT, MARCELO; TRABLY, ERIC. Biogas sequestration from the headspace of a fermentative system enhances hydrogen production rate and yield. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 45, n. 19, p. 11011-11023, . (13/15665-8, 15/21650-9)
DOS SANTOS, CARLA ELOISA DINIZ; COSTA, RACHEL BIANCALANA; RABELO, CAMILA ABREU BORGES SILVA; FERRAZ JUNIOR, ANTONIO DJALMA NUNES; PERSINOTI, GABRIELA FELIX; POZZI, ELOISA; FORESTI, EUGENIO; DAMIANOVIC, MARCIA HELENA RISSATO ZAMARIOLLI. Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal. Bioprocess and Biosystems Engineering, v. 44, n. 9, p. 1841-1851, . (13/15665-8, 15/21650-9)
KOYAMA, MIRIAN HARUMI; ARAUJO JUNIOR, MOACIR MESSIAS; ZAIAT, MARCELO; NUNES FERRAZ JUNIOR, ANTONIO DJALMA. Kinetics of thermophilic acidogenesis of typical Brazilian sugarcane vinasse. ENERGY, v. 116, p. 7-pg., . (09/15984-0, 13/15665-8, 15/21650-9)
KOYAMA, MIRIAN HARUMI; ARAUJO JUNIOR, MOACIR MESSIAS; ZAIAT, MARCELO; NUNES FERRAZ JUNIOR, ANTONIO DJALMA. Kinetics of thermophilic acidogenesis of typical Brazilian sugarcane vinasse. ENERGY, v. 116, n. 1, p. 1097-1103, . (09/15984-0, 15/21650-9, 13/15665-8)
FUESS, LUCAS TADEU; MAZINE KIYUNA, LUMA SAYURI; NUNES FERRAZ JUNIOR, ANTONIO DJALMA; PERSINOTI, GABRIELA FELIX; SQUINA, FABIO MARCIO; GARCIA, MARCELO LOUREIRO; ZAIAT, MARCELO. Thermophilic two-phase anaerobic digestion using an innovative fixed-bed reactor for enhanced organic matter removal and bioenergy recovery from sugarcane vinasse. APPLIED ENERGY, v. 189, p. 480-491, . (09/15984-0, 12/15606-9, 15/21650-9, 13/15665-8)
FUESS, LUCAS TADEU; NUNES FERRAZ, JR., ANTONIO DJALMA; MACHADO, CARLA BOTELHO; ZAIAT, MARCELO. Temporal dynamics and metabolic correlation between lactate-producing and hydrogen-producing bacteria in sugarcane vinasse dark fermentation: The key role of lactate. Bioresource Technology, v. 247, p. 426-433, . (09/15984-0, 12/15606-9, 15/21650-9, 13/15665-8)

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