Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal

Author(s)

dos Santos, Carla Eloisa Diniz ^{[1, 2]} ; Costa, Rachel Biancalana ^[3] ; Rabelo, Camila Abreu Borges Silva ^[2] ; Ferraz Junior, Antonio Djalma Nunes ^{[4, 5]} ; Persinoti, Gabriela Felix ^[4] ; Pozzi, Eloisa ^[2] ; Foresti, Eugenio ^[2] ; Damianovic, Marcia Helena Rissato Zamariolli ^[2]

Affiliation

^[1] Fed Univ Triangulo Mineiro UFTM, Environm Engn Dept, Av Dr Randolfo Borges Jr 1250, BR-38064200 Uberaba, MG - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Biol Proc Lab, Av Joao Dagnone 1100, BR-13563120 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Organ Chem, R Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[4] Brazilian Biorenewables Natl Lab LNBR CNPEM, Rua Giuseppe Maximo Scolfaro 10-000, BR-13083970 Campinas, SP - Brazil

^[5] Inst Invest Biol Clemente Estable, Lab Ecol Microbiana, Dept Bioquim & Genom Microbiana, Av Italia 3318, Montevideo - Uruguay

• Document type: Journal article
• Source: Bioprocess and Biosystems Engineering; v. 44, n. 9, p. 1841-1851, SEP 2021.
• Web of Science Citations: 0

Abstract

Biomass samples from a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) were analyzed to investigate the bacterial community shift along with the changes in the C/N ratio. The C/N ratios tested were 7.6 +/- 1.0 (LNC) and 2.9 +/- 0.4 (HNC). The massive sequencing analyses revealed that the microbial community adjusted itself to different organic and nitrogenous applied loads, with no harm to reactor performance regarding COD and Total-N removal. Under LNC, conventional nitrification and heterotrophic denitrification steered the process, as indicated by the detection of microorganisms affiliated with Nitrosomonadaceae, Nitrospiraceae, and Rhodocyclaceae families. However, under HNC, the C/N ratio strongly affected the microbial community, resulting in the prevalence of members of Saprospiraceae, Chitinophagaceae, Xanthomonadaceae, Comamonadaceae, Bacillaceae, and Planctomycetaceae. These families include bacteria capable of using organic matter derived from cell lysis, ammonia-oxidizers under low DO, heterotrophic nitrifiers-aerobic denitrifiers, and non-isolated strains of Anammox. The DO profile confirmed that the stratification in aerobic, anoxic, and anaerobic zones enabled the establishment of different nitrogen degradation pathways, including the Anammox. (AU)

• FAPESP's process: 13/15665-8 - Structure and functional dynamics of microbial community of anaerobic digesters applied to energy recovery and environmental framework of wastes generated in bioethanol production of 1st and 2nd generation
• Grantee: Antônio Djalma Nunes Ferraz Júnior
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/21650-9 - Applied metagenomics for biofuels development and environmental sustainability in biorefineries based on sugarcane
• Grantee: Antônio Djalma Nunes Ferraz Júnior
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor