Hacking biofilm developed in a structured-bed reactor (SBRRIA) with integrated processes of nitrogen and organic matter removal

Autor(es)

dos Santos, Carla Eloisa Diniz ^{[1, 2]} ; Costa, Rachel Biancalana ^[3] ; Rabelo, Camila Abreu Borges Silva ^[2] ; Ferraz Junior, Antonio Djalma Nunes ^{[4, 5]} ; Persinoti, Gabriela Felix ^[4] ; Pozzi, Eloisa ^[2] ; Foresti, Eugenio ^[2] ; Damianovic, Marcia Helena Rissato Zamariolli ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Triangulo Mineiro UFTM, Environm Engn Dept, Av Dr Randolfo Borges Jr 1250, BR-38064200 Uberaba, MG - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Biol Proc Lab, Av Joao Dagnone 1100, BR-13563120 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, Dept Biochem & Organ Chem, R Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

^[4] Brazilian Biorenewables Natl Lab LNBR CNPEM, Rua Giuseppe Maximo Scolfaro 10-000, BR-13083970 Campinas, SP - Brazil

^[5] Inst Invest Biol Clemente Estable, Lab Ecol Microbiana, Dept Bioquim & Genom Microbiana, Av Italia 3318, Montevideo - Uruguay

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 44, n. 9, p. 1841-1851, SEP 2021.
• Citações Web of Science: 0

Resumo

Biomass samples from a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) were analyzed to investigate the bacterial community shift along with the changes in the C/N ratio. The C/N ratios tested were 7.6 +/- 1.0 (LNC) and 2.9 +/- 0.4 (HNC). The massive sequencing analyses revealed that the microbial community adjusted itself to different organic and nitrogenous applied loads, with no harm to reactor performance regarding COD and Total-N removal. Under LNC, conventional nitrification and heterotrophic denitrification steered the process, as indicated by the detection of microorganisms affiliated with Nitrosomonadaceae, Nitrospiraceae, and Rhodocyclaceae families. However, under HNC, the C/N ratio strongly affected the microbial community, resulting in the prevalence of members of Saprospiraceae, Chitinophagaceae, Xanthomonadaceae, Comamonadaceae, Bacillaceae, and Planctomycetaceae. These families include bacteria capable of using organic matter derived from cell lysis, ammonia-oxidizers under low DO, heterotrophic nitrifiers-aerobic denitrifiers, and non-isolated strains of Anammox. The DO profile confirmed that the stratification in aerobic, anoxic, and anaerobic zones enabled the establishment of different nitrogen degradation pathways, including the Anammox. (AU)

• Processo FAPESP: 13/15665-8 - Estrutura e dinâmica funcional da comunidade microbiana de digestores anaeróbios, aplicados à recuperação de energia e adequação ambiental de resíduos gerados no processo produtivo do bioetanol de 1ª e 2ª geração
• Beneficiário: Antônio Djalma Nunes Ferraz Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/21650-9 - Metagenômica aplicada ao desenvolvimento de biocombustíveis e ao desenvolvimento sustentável das biorrefinarias baseadas na cana-de-açúcar
• Beneficiário: Antônio Djalma Nunes Ferraz Júnior
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado