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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Phage-Like Plasmid Carrying bla(KPC-2) Gene in Carbapenem-Resistant Pseudomonas aeruginosa

Texto completo
Autor(es):
Galetti, Renata [1] ; Andrade, Leonardo Neves [1] ; Varani, Alessandro M. [2] ; Costa Darini, Ana Lucia [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto - Brazil
[2] Sao Paulo State Univ Julio de Mesquita Filho UNES, Sch Agr & Vet Studies, Jaboticabal - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 10, MAR 20 2019.
Citações Web of Science: 0
Resumo

Background: Lateral gene transfer plays a central role in the dissemination of carbapenem resistance in bacterial pathogens associated with nosocomial infections, mainly Enterobacteriaceae and Pseudomonas aeruginosa. Despite their clinical significance, there is little information regarding the mobile genetic elements and mechanism of acquisition and propagation of lateral genes in P. aeruginosa, and they remain largely unknown. Objectives: The present study characterized the genetic context of bla(KPC-2) in carbapenem-resistant P. aeruginosa strain BH9. Methods: Pseudomonas aeruginosa BH9 sequencing was performed using the long-read PacBio SMRT platform and the Ion Proton System. De novo assembly was carried out using the SMRT pipeline and Canu, and gene prediction and annotation were performed using Prokka and RAST. Results: Pseudomonas aeruginosa BH9 exhibited a 7.1 Mb circular chromosome. However, the bla(KPC-2) gene is located in an additional contig composed by a small plasmid pBH6 from P. aeruginosa strain BH6 and several phage-related genes. Further analysis revealed that the beginning and end of the contig contain identical sequences, supporting a circular plasmid structure. This structure spans 41,087 bp, exhibiting all the Mu-like phage landmarks. In addition, 5-bp direct repeats (GGATG) flanking the pBH6 ends were found, strongly indicating integration of the Mu-like phage into the pBH6 plasmid. Mu phages are commonly found in P. aeruginosa. However, for the first time showing a potential impact in shaping the vehicles of the dissemination of antimicrobial (e.g., plasmid pBH6) resistance genes in the Pseudomonas genus. Conclusion: pBH6 captured the Mu-like Phage BH9, creating a co-integrate pBH6::Phage BH9, and this phage-plasmid complex may represent novel case of a phage-like plasmid. (AU)

Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
Beneficiário:Ana Lúcia da Costa Darini
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/11728-0 - Sequenciamento completo de plasmídeos carreando genes de resistência a antibióticos em bactérias de interesse e análise do genoma de linhagens de Pseudomonas aeruginosa produtoras de SPM-1 e de KPC-2 por sequenciamento em larga escala
Beneficiário:Renata Galetti
Linha de fomento: Bolsas no Brasil - Pós-Doutorado