Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Autor(es)

Romano de Oliveira Goncalves, Priscila Jane ^[1] ; Hume, Carmen C. Denman ^[2] ; Ferreira, Almir Jose ^[1] ; Tsui, Sarina ^[1] ; Brocchi, Marcelo ^[3] ; Wren, Brendan W. ^[2] ; Araujo, Welington Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[2] London Sch Hyg & Trop Med, Dept Pathogen Mol Biol, London WC1E 7HT - England

^[3] Univ Estadual Campinas, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, APR 2 2019.
• Citações Web of Science: 0

Resumo

Burkholderia seminalis strain TC3.4.2R3 is an endophytic bacterium isolated from sugarcane roots that produces antimicrobial compounds, facilitating its ability to act as a biocontrol agent against phytopathogenic bacteria. In this study, we investigated the thermoregulation of B. seminalis TC3.4.2R3 at 28 degrees C (environmental stimulus) and 37 degrees C (host-associated stimulus) at the transcriptional and phenotypic levels. The production of biofilms and exopolysaccharides such as capsular polysaccharides and the biocontrol of phytopathogenic fungi were enhanced at 28 degrees C. At 37 degrees C, several metabolic pathways were activated, particularly those implicated in energy production, stress responses and the biosynthesis of transporters. Motility, growth and virulence in the Galleria mellonella larvae infection model were more significant at 37 degrees C. Our data suggest that the regulation of capsule expression could be important in virulence against G. mellonella larvae at 37 degrees C. In contrast, B. seminalis TC3.4.2R3 failed to cause death in infected BALB/c mice, even at an infective dose of 10(7) CFU.mL(-1). We conclude that temperature drives the regulation of gene expression in B. seminalis during its interactions with the environment. (AU)

• Processo FAPESP: 17/50447-2 - Burkholderia spp. na cultura de cana-de-açúcar: a relação entre a produção de antifúngico, resistência intrínseca a antimicrobianos e o controle de pragas
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/24217-6 - Micro-organismos endofíticos: identificação de genes envolvidos em interações microbianas
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/03426-9 - Caracterização do cluster wcb de B. seminalis TC3.4.2R3 e do seu papel na síntese de antimicrobianos
• Beneficiário: Priscila Jane Romano de Oliveira Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/24375-6 - Caracterização do cluster wcb de b. seminalis TC3.4.2R3 e do seu papel na síntese de antimicrobianos
• Beneficiário: Priscila Jane Romano de Oliveira Gonçalves
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/12510-4 - Micro-organismos endofíticos e a planta hospedeira: genes e metabólitos envolvidos na interação
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular